About Gene List

PF3D7_1031400 (OTU)

Genome location: Pf3D7_10_v3:1,263,906..1,269,524(+)

Genome classification: Core

Function and Localization

Product Description: OTU-like cysteine protease

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.22.- (Cysteine endopeptidases.)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0005829 cytosol
Component GO:0031982 vesicle
Function GO:0008233 peptidase activity
Function GO:0004843 thiol-dependent deubiquitinase
Process GO:1903060 negative regulation of protein lipidation
Process GO:0016579 protein deubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring possibly expressed nd nd
Trophozoite possibly expressed nd nd
Schizont expressed nd nd
Gametocyte expressed nd nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1031400.2

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.676 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0515350)

  • Relative Growth Rate: 1.04 ± 0.07
  • Confidence: 6.80

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0515350)

Modification: Disrupted | RMgm-2008

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A039

"Best" AlphaFill ligand hit: 3CN (3-aminopropane, Local RMSD=0.76) with 4HXD (Global RMSD=7.61)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.4.22.- Ex: staphylococcal cysteine proteinase NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT...
3.4.22.- Ex: staphylococcal cysteine proteinase NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104209

Most Similar Human Ortholog: Q5T2D3

TM-align score: 0.40 | RMSD: 4.63

Seq Identity: 0.20 | Length: 190 / 938

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000169914 OTU deubiquitinase 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 9 53 37 56
disruptive 29 114 75 173
missense 26 99 65 131

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 16 116 44 64
disruptive 56 295 137 287
missense 42 224 108 170

PlasmoDB Total SNPs: 273

Non-coding: 166 | Synonymous: 57 | Nonsynonymous: 50 | Stop Codon: 0

Protein Information

Protein Length: 938 | Molecular Weight (kDa): 111.71

UniProt ID(s): A0A143ZXT6

PDB ID(s): None

Isoelectric Point: 8.6

Protein Domain Annotations:

Source Family ID Description
InterPro IPR003323 OTU domain
InterPro IPR038765 Papain-like cysteine peptidase superfamily
PFam PF02338 OTU domain
Superfamily SSF54001 Papain-like cysteine peptidase superfamily

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097