PF3D7_1031400 (OTU)

Genome location: Pf3D7_10_v3:1,263,906..1,269,524(+)

Genome classification: Core

Function and Localization

Product Description: OTU-like cysteine protease

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.22.- (Cysteine endopeptidases.)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005829	cytosol
Component	GO:0031982	vesicle
Function	GO:0008233	peptidase activity
Function	GO:0004843	thiol-dependent deubiquitinase
Process	GO:1903060	negative regulation of protein lipidation
Process	GO:0016579	protein deubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	nd	nd
Trophozoite	possibly expressed	nd	nd
Schizont	expressed	nd	nd
Gametocyte	expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1031400.2

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.676 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0515350)

Relative Growth Rate: 1.04 ± 0.07
Confidence: 6.80

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0515350)

Modification: Disrupted | RMgm-2008

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A039

"Best" AlphaFill ligand hit: 3CN (3-aminopropane, Local RMSD=0.76) with 4HXD (Global RMSD=7.61)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.22.-	Ex: staphylococcal cysteine proteinase	NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT...
3.4.22.-	Ex: staphylococcal cysteine proteinase	NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104209

Most Similar Human Ortholog: Q5T2D3

TM-align score: 0.40 | RMSD: 4.63

Seq Identity: 0.20 | Length: 190 / 938

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000169914	OTU deubiquitinase 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	53	37	56
disruptive	29	114	75	173
missense	26	99	65	131

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	16	116	44	64
disruptive	56	295	137	287
missense	42	224	108	170

PlasmoDB Total SNPs: 273

Non-coding: 166 | Synonymous: 57 | Nonsynonymous: 50 | Stop Codon: 0

Protein Information

Protein Length: 938 | Molecular Weight (kDa): 111.71

UniProt ID(s): A0A143ZXT6

PDB ID(s): None

Isoelectric Point: 8.6

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003323	OTU domain
InterPro	IPR038765	Papain-like cysteine peptidase superfamily
PFam	PF02338	OTU domain
Superfamily	SSF54001	Papain-like cysteine peptidase superfamily