Genome location: Pf3D7_10_v3:1,269,537..1,274,166(+)
Genome classification: Core
Product Description: ATP-dependent DNA helicase DDX3X
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003678 | DNA helicase activity |
| Process | GO:0000398 | mRNA splicing, via spliceosome |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.36 | 0.82 |
| Trophozoite | expressed | 0.63 | 0.64 |
| Schizont | expressed | 0.28 | 0.82 |
| Gametocyte | expressed | 0.73 | 0.60 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1031500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.988 | #Insertions: 5
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ90
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.05) with 5JCH (Global RMSD=1.75)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 37 | 24 | 64 |
| disruptive | 42 | 139 | 66 | 153 |
| missense | 19 | 102 | 51 | 121 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 98 | 42 | 61 |
| disruptive | 85 | 300 | 142 | 288 |
| missense | 39 | 220 | 101 | 168 |
PlasmoDB Total SNPs: 307
Non-coding: 192 | Synonymous: 60 | Nonsynonymous: 54 | Stop Codon: 1
Protein Length: 917 | Molecular Weight (kDa): 107.8
UniProt ID(s): Q8IJ90
PDB ID(s): None
Isoelectric Point: 8.13
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000629 | ATP-dependent RNA helicase DEAD-box, conserved site |
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 34265466 | Plasmodium falciparum DDX3X is a nucleocytoplasmic protein and requiresN-terminal for DNA helicase activity. | Sourabh S, Yasmin R, Tuteja R | 10.1016/j.parint.2021.102420 |