Genome location: Pf3D7_10_v3:1,302,298..1,305,315(-)
Genome classification: Core
Product Description: phosphatidylinositol N-acetylglucosaminyltransferase subunit A, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.4.1.198 (Phosphatidylinositol N-acetylglucosaminyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0003824 | catalytic activity |
| Function | GO:0017176 | phosphatidylinositol N-acetylglucosaminyltransferase activity |
| Process | GO:0006506 | GPI anchor biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.24 | 0.84 |
| Schizont | expressed | 0.14 | 0.90 |
| Gametocyte | not expressed | 0.14 | 0.92 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1032400
Resistome Missense Mutations: R221K
Resistome Compounds with Missense Mutations: MMV665794
Resistome # Samples with Disruptive Mutations: 4 (3 missense, 3 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.798 | MFS: -2.212 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ83
"Best" AlphaFill ligand hit: UPG (uridine-5'-diphosphate-glucose, Local RMSD=1.68) with 4XSR (Global RMSD=6.19)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.1.198 | phosphatidylinositol N-acetylglucosaminyltransferase | iodoacetic acidN-ethylmaleimideSH-alkylating reagentsp-chloromercuriphenylsulfonic acid |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100580
Most Similar Human Ortholog: P37287
TM-align score: 0.91 | RMSD: 1.49
Seq Identity: 0.47 | Length: 431 / 461
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000165195 | phosphatidylinositol glycan anchor biosynthesis class A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 22 | 21 | 31 |
| disruptive | 4 | 11 | 5 | 23 |
| missense | 4 | 10 | 5 | 20 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 55 | 32 | 24 |
| disruptive | 6 | 30 | 20 | 67 |
| missense | 6 | 21 | 16 | 46 |
PlasmoDB Total SNPs: 124
Non-coding: 108 | Synonymous: 14 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 461 | Molecular Weight (kDa): 53.113
UniProt ID(s): Q8IJ83
PDB ID(s): None
Isoelectric Point: 9.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001296 | Glycosyl transferase, family 1 |
| InterPro | IPR013234 | PIGA, GPI anchor biosynthesis |
| PFam | PF00534 | Glycosyl transferase, family 1 |
| PFam | PF08288 | PIGA, GPI anchor biosynthesis |
| Superfamily | SSF53756 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12117963 | Genes for glycosylphosphatidylinositol toxin biosynthesis in Plasmodiumfalciparum. | Delorenzi M, Sexton A, ..., Schofield L | 10.1128/IAI.70.8.4510-4522.2002 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |