Genome location: Pf3D7_10_v3:1,323,813..1,331,955(-)
Genome classification: Core
Product Description: S-adenosylmethionine decarboxylase/ornithine decarboxylase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.1.1.17 (Ornithine decarboxylase);4.1.1.50 (Adenosylmethionine decarboxylase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0004014 | adenosylmethionine decarboxylase activity |
| Function | GO:0004586 | ornithine decarboxylase activity |
| Process | GO:0006596 | polyamine biosynthetic process |
| Process | GO:0033387 | putrescine biosynthetic process from ornithine |
| Process | GO:0009445 | putrescine metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 2.29 | 0.17 |
| Trophozoite | expressed | 2.42 | 0.11 |
| Schizont | expressed | 1.97 | 0.18 |
| Gametocyte | expressed | 1.04 | 0.49 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1033100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.18 | MFS: -2.799 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0516900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_0518000)
Modification: Disrupted | RMgm-1496
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ77
"Best" AlphaFill ligand hit: N2P (pentane-1,5-diamine, Local RMSD=0.13) with 5GJP (Global RMSD=2.12)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100277
Most Similar Human Ortholog: P11926
TM-align score: 0.82 | RMSD: 2.78
Seq Identity: 0.36 | Length: 408 / 1434
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000115758 | ornithine decarboxylase 1 |
Protein Length: 1434 | Molecular Weight (kDa): 168.17
UniProt ID(s): O15696, O15869, Q8IJ77, Q9U4U6, Q9U8D4, Q9Y1L5
PDB ID(s): None
Isoelectric Point: 6.31
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000183 | Ornithine/DAP/Arg decarboxylase |
| InterPro | IPR001985 | S-adenosylmethionine decarboxylase |
| InterPro | IPR002433 | Ornithine decarboxylase |
| InterPro | IPR009006 | Alanine racemase/group IV decarboxylase, C-terminal |
| InterPro | IPR016067 | S-adenosylmethionine decarboxylase, core |
| InterPro | IPR022643 | Orn/DAP/Arg decarboxylase 2, C-terminal |
| InterPro | IPR022644 | Orn/DAP/Arg decarboxylase 2, N-terminal |
| InterPro | IPR029066 | PLP-binding barrel |
| PFam | PF00278 | Orn/DAP/Arg decarboxylase 2, C-terminal |
| PFam | PF01536 | S-adenosylmethionine decarboxylase |
| PFam | PF02784 | Orn/DAP/Arg decarboxylase 2, N-terminal |
| Superfamily | SSF50621 | Alanine racemase/group IV decarboxylase, C-terminal |
| Superfamily | SSF51419 | PLP-binding barrel |
| Superfamily | SSF56276 | S-adenosylmethionine decarboxylase, core |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 100 | 48 | 121 |
| disruptive | 110 | 265 | 103 | 241 |
| missense | 70 | 195 | 82 | 193 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 42 | 183 | 70 | 109 |
| disruptive | 181 | 456 | 180 | 326 |
| missense | 117 | 344 | 139 | 205 |
PlasmoDB Total SNPs: 513
Non-coding: 291 | Synonymous: 87 | Nonsynonymous: 135 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10713131 | In the human malaria parasite Plasmodium falciparum, polyamines are synthesizedby a bifunctional ornithine decarboxylase, S-adenosylmethionine decarboxylase. | Muller S, Da'dara A, ..., Walter RD | 10.1074/jbc.275.11.8097 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16257247 | Novel properties of malarial S-adenosylmethionine decarboxylase as revealed bystructural modelling. | Wells GA, Birkholtz LM, ..., Louw AI | 10.1016/j.jmgm.2005.09.011 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19073607 | Co-inhibition of Plasmodium falciparum S-adenosylmethioninedecarboxylase/ornithine decarboxylase reveals perturbation-specific compensatorymechanisms by transcriptome, proteome, and metabolome analyses. | van Brummelen AC, Olszewski KL, ..., Birkholtz LM | 10.1074/jbc.M807085200 |
| 21803076 | Biochemical characterisation and novel classification of monofunctionalS-adenosylmethionine decarboxylase of Plasmodium falciparum. | Williams M, Sprenger J, ..., Birkholtz LM | https://pubmed.ncbi.nlm.nih.gov/21803076/ |
| 24524072 | Vitamin B6-dependent enzymes in the human malaria parasite Plasmodium falciparum:a druggable target? | Kronenberger T, Lindner J, ..., Wrenger C | 10.1155/2014/108516 |
| 27387533 | Plasmodium AdoMetDC/ODC bifunctional enzyme is essential for male sexual stagedevelopment and mosquito transmission | Hart RJ, Ghaffar A, ..., Aly AS | 10.1242/bio.016352 |
| 12974675 | Parasite-specific inserts in the bifunctional S-adenosylmethioninedecarboxylase/ornithine decarboxylase of Plasmodium falciparum modulate catalyticactivities and domain interactions | Birkholtz LM, Wrenger C, ..., Louw AI | 10.1042/BJ20030614 |
| 18455248 | Assessing the polyamine metabolism of Plasmodium falciparum as chemotherapeutictarget | Muller IB, Das Gupta R, ..., Walter RD | 10.1016/j.molbiopara.2008.03.008 |