Genome location: Pf3D7_10_v3:1,339,140..1,341,120(+)
Genome classification: Core
Product Description: haloacid dehalogenase-like hydrolase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.8.1.- (In C-halide compounds.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0050308 | sugar-phosphatase activity |
| Process | GO:0016311 | dephosphorylation |
| Process | GO:0010323 | negative regulation of isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.31 | 0.85 |
| Trophozoite | expressed | 0.64 | 0.67 |
| Schizont | expressed | 0.04 | 0.97 |
| Gametocyte | not expressed | 0.08 | 0.95 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1033400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.767 | MFS: -2.311 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1441100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0517200)
Modification: Disrupted | RMgm-2018
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ74
"Best" AlphaFill ligand hit: G3H (glyceraldehyde-3-phosphate, Local RMSD=0.24) with 4ZEX (Global RMSD=0.27)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.8.1.- | Ex: (S)-2-haloacid dehalogenase | NEMSDSATPCoAPCMBHgCl2ZnSO4CuSO4FeSO4MnSO4HgSO4RbCl2... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 12 | 6 | 10 |
| disruptive | 5 | 13 | 5 | 24 |
| missense | 5 | 13 | 5 | 20 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 25 | 7 | 10 |
| disruptive | 9 | 32 | 16 | 53 |
| missense | 9 | 28 | 13 | 34 |
PlasmoDB Total SNPs: 56
Non-coding: 38 | Synonymous: 12 | Nonsynonymous: 6 | Stop Codon: 0
Protein Length: 288 | Molecular Weight (kDa): 32.842
UniProt ID(s): Q8IJ74
PDB ID(s): 4QJB, 4ZEV, 4ZEW, 4ZEX
Isoelectric Point: 5.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000150 | Cof family |
| InterPro | IPR006379 | HAD-superfamily hydrolase, subfamily IIB |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF08282 | N/A |
| Superfamily | SSF56784 | HAD-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 25058848 | A sugar phosphatase regulates the methylerythritol phosphate (MEP) pathway inmalaria parasites. | Guggisberg AM, Park J, ..., Odom AR | 10.1038/ncomms5467 |