PF3D7_1033800

Genome location: Pf3D7_10_v3:1,351,197..1,353,284(+)

Genome classification: Core

Function and Localization

Product Description: plasmepsin VII

SignalP Peptide: MNKNIIQIYLFVFILLLKQHIVIL

# Transmembrane Domains: 1

EC Numbers: 3.4.23.- (Aspartic endopeptidases.)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004190	aspartic-type endopeptidase activity
Process	GO:0006508	proteolysis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.01	1.00
Trophozoite	possibly expressed	0.02	0.99
Schizont	possibly expressed	0.01	0.99
Gametocyte	expressed	0.44	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1033800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.141 | MFS: -2.891 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0517600)

Relative Growth Rate: 1.00 ± 0.10
Confidence: 6.06

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0517600)

Modification: Disrupted | RMgm-2020

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJ71

"Best" AlphaFill ligand hit: CPS (chaps, Local RMSD=0.02) with 7VE2 (Global RMSD=3.57)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.23.-	Ex: renin	I2YYKCNSDSIAANBSDANEDTAPI-3DMSOPCMBTLCK...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q8IM16	Plasmepsin IV [1-448]		HOGENOM	phenyl-substituted sulfonamides, 6dphenyl-substituted sulfonamides, 6eCHEMBL588662US9353089, 117...
O60989	Plasmepsin IV, putative	Plasmodium vivax	HOGENOM	CHEMBL575115CHEMBL584189CHEMBL575150CHEMBL584190...
P20142	Gastricsin	Homo sapiens	OMA	CHEMBL313773CHEMBL418948CHEMBL287423CHEMBL62380...
P04073	Gastricsin	Rattus norvegicus	OMA	CHEMBL28888CHEMBL424222CHEMBL28738

Orthology Information

Ortholog Group (OrthoMCL): OG6_139465

No human ortholog(s)

Protein Information

Protein Length: 450 | Molecular Weight (kDa): 52.328

UniProt ID(s): Q2KNY9, Q8IJ71

PDB ID(s): None

Isoelectric Point: 8.33

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001461	Aspartic peptidase A1 family
InterPro	IPR021109	Aspartic peptidase domain superfamily
InterPro	IPR033121	Peptidase family A1 domain
InterPro	IPR034164	Pepsin-like domain
PFam	PF00026	Peptidase family A1 domain
Superfamily	SSF50630	Aspartic peptidase domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	30	20	45
disruptive	5	50	31	71
missense	5	50	30	69

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	62	21	38
disruptive	9	109	68	106
missense	9	100	61	75

PlasmoDB Total SNPs: 109

Non-coding: 82 | Synonymous: 14 | Nonsynonymous: 13 | Stop Codon: 0