PF3D7_1033900

Genome location: Pf3D7_10_v3:1,354,427..1,357,118(-)

Genome classification: Core

Function and Localization

Product Description: ubiquitin-conjugating enzyme E2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.3.2.23 (E2 ubiquitin-conjugating enzyme)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft
Component	GO:0005634	nucleus
Function	GO:0061631	ubiquitin conjugating enzyme activity
Function	GO:0004842	ubiquitin-protein transferase activity
Process	GO:0000209	protein polyubiquitination
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.13	0.94
Trophozoite	expressed	0.57	0.71
Schizont	expressed	1.07	0.54
Gametocyte	expressed	1.66	0.27

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1033900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.943 | MFS: -2.173 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0517700)

Relative Growth Rate: 1.01 ± 0.06
Confidence: 6.87

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0517700)

Modification: Disrupted | RMgm-2021

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJ70

"Best" AlphaFill ligand hit: BU3 ((r,r)-2,3-butanediol, Local RMSD=0.04) with 1Z2U (Global RMSD=3.69)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.2.23	E2 ubiquitin-conjugating enzyme	CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P63146	Ubiquitin-conjugating enzyme E2 B		OrthoDB	CHEMBL3787660
P51965	Ubiquitin-conjugating enzyme E2 E1		OrthoDB	US9796725, Compound I-001US9796725, Compound I-098US9796725, Compound I-003US9796725, Compound I-004...
P61088	Ubiquitin-conjugating enzyme E2 N	Homo sapiens	OrthoDB	MLS000074439MLS000032400MLS000041097MLS000055488...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102420

Most Similar Human Ortholog: H7C4M9

TM-align score: 0.89 | RMSD: 0.96

Seq Identity: 0.48 | Length: 62 / 191

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000186591	ubiquitin conjugating enzyme E2 H

Protein Information

Protein Length: 191 | Molecular Weight (kDa): 21.571

UniProt ID(s): Q8IJ70

PDB ID(s): 2ONU

Isoelectric Point: 4.29

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000608	Ubiquitin-conjugating enzyme E2
InterPro	IPR016135	Ubiquitin-conjugating enzyme/RWD-like
InterPro	IPR023313	Ubiquitin-conjugating enzyme, active site
PFam	PF00179	Ubiquitin-conjugating enzyme E2
Superfamily	SSF54495	Ubiquitin-conjugating enzyme/RWD-like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	0
disruptive	0	0	0	0
missense	0	0	0	0

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	0
disruptive	0	0	0	1
missense	0	0	0	0

PlasmoDB Total SNPs: 196

Non-coding: 192 | Synonymous: 4 | Nonsynonymous: 0 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
31631203	A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum.	Das D, Krishnan SR, Roy A, Bulusu G	10.1039/c9mo00124g