Genome location: Pf3D7_10_v3:1,366,770..1,369,652(-)
Genome classification: Core
Product Description: flavoprotein subunit of succinate dehydrogenase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.3.5.1 (Succinate dehydrogenase (quinone))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005749 | mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0009055 | electron transfer activity |
| Function | GO:0050660 | flavin adenine dinucleotide binding |
| Function | GO:0016491 | oxidoreductase activity |
| Function | GO:0008177 | succinate dehydrogenase (ubiquinone) activity |
| Function | GO:0000104 | succinate dehydrogenase activity |
| Process | GO:0006121 | mitochondrial electron transport, succinate to ubiquinone |
| Process | GO:0006099 | tricarboxylic acid cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.14 | 0.91 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1034400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.295 | MFS: -2.831 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0518200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0518200)
Modification: Disrupted | RMgm-2024
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ66
"Best" AlphaFill ligand hit: HEM (heme, Local RMSD=0.18) with 1Y0P (Global RMSD=1.76)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.3.5.1 | succinate dehydrogenase | oxalateMaleatefumaratemalonaterotenonecarboxinsiccaninboscalidfenfurammepronilclozapinebenodanil... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P31040 | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial | OrthoDB | cid_441401 |
Ortholog Group (OrthoMCL): OG6_100447
Most Similar Human Ortholog: P31040
TM-align score: 0.94 | RMSD: 1.09
Seq Identity: 0.65 | Length: 598 / 631
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000073578 | succinate dehydrogenase complex flavoprotein subunit A |
| ENSG00000286001 | novel protein |
Protein Length: 631 | Molecular Weight (kDa): 70.695
PDB ID(s): None
Isoelectric Point: 8.24
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003952 | Fumarate reductase/succinate dehydrogenase, FAD-binding site |
| InterPro | IPR003953 | FAD-dependent oxidoreductase 2, FAD binding domain |
| InterPro | IPR011281 | Succinate dehydrogenase, flavoprotein subunit |
| InterPro | IPR014006 | Succinate dehydrogenase/fumarate reductase, flavoprotein subunit |
| InterPro | IPR015939 | Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal |
| InterPro | IPR027477 | Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain superfamily |
| InterPro | IPR036188 | FAD/NAD(P)-binding domain superfamily |
| InterPro | IPR037099 | Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain superfamily |
| PFam | PF00890 | FAD-dependent oxidoreductase 2, FAD binding domain |
| PFam | PF02910 | Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal |
| Superfamily | SSF46977 | Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain superfamily |
| Superfamily | SSF51905 | FAD/NAD(P)-binding domain superfamily |
| Superfamily | SSF56425 | Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 39 | 24 | 39 |
| disruptive | 4 | 16 | 17 | 38 |
| missense | 4 | 16 | 17 | 34 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 87 | 28 | 25 |
| disruptive | 6 | 47 | 26 | 76 |
| missense | 5 | 44 | 23 | 59 |
PlasmoDB Total SNPs: 130
Non-coding: 120 | Synonymous: 7 | Nonsynonymous: 3 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 22698672 | Toward understanding the role of mitochondrial complex II in theintraerythrocytic stages of Plasmodium falciparum: gene targeting of the Fpsubunit. | Tanaka TQ, Hirai M, Watanabe Y, Kita K | 10.1016/j.parint.2012.06.002 |
| 25843709 | Genetic investigation of tricarboxylic acid metabolism during the Plasmodiumfalciparum life cycle. | Ke H, Lewis IA, ..., Vaidya AB | 10.1016/j.celrep.2015.03.011 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 38079448 | Functional and biochemical characterization of the Toxoplasma gondii succinatedehydrogenase complex | Silva MF, Douglas K, ..., Sheiner L | 10.1371/journal.ppat.1011867 |