Genome location: Pf3D7_10_v3:1,383,164..1,386,554(+)
Genome classification: Core
Product Description: methionine--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.10 (Methionine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004825 | methionine-tRNA ligase activity |
| Process | GO:0006431 | methionyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.67 | 0.69 |
| Trophozoite | expressed | 1.41 | 0.35 |
| Schizont | expressed | 0.41 | 0.76 |
| Gametocyte | expressed | 0.47 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1034900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.123 | MFS: -2.954 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ60
"Best" AlphaFill ligand hit: CXS (3-cyclohexyl-1-propylsulfonic acid, Local RMSD=0.32) with 2X1L (Global RMSD=3.97)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.10 | methionine-tRNA ligase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q38C91 | Methionine--tRNA ligase | Trypanosoma brucei brucei (strain 927/4 GUTat10.1) | OrthoDB, OrthoMCL | MLS000073857GNF-Pf-4478MLS000041737MLS000058063... |
| Q96GW9 | Methionine--tRNA ligase, mitochondrial | OrthoDB | CHEMBL4791458CHEMBL4781504CHEMBL4742903CHEMBL4777699... | |
| Q8NY00 | Methionine--tRNA ligase | Staphylococcus aureus (strain MW2) | OrthoMCL BLAST | CHEMBL425706CHEMBL361649CHEMBL182821CHEMBL361747... |
Ortholog Group (OrthoMCL): OG6_101119
Most Similar Human Ortholog: Q96GW9
TM-align score: 0.80 | RMSD: 3.73
Seq Identity: 0.31 | Length: 531 / 889
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000247626 | methionyl-tRNA synthetase 2, mitochondrial |
Protein Length: 889 | Molecular Weight (kDa): 104.168
UniProt ID(s): Q8IJ60
PDB ID(s): None
Isoelectric Point: 7.61
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR009080 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| InterPro | IPR014758 | Methionyl-tRNA synthetase |
| InterPro | IPR015413 | Methionyl/Leucyl tRNA synthetase |
| InterPro | IPR033911 | Methioninyl-tRNA synthetase core domain |
| InterPro | IPR036282 | Glutathione S-transferase, C-terminal domain superfamily |
| PFam | PF09334 | Methionyl/Leucyl tRNA synthetase |
| Superfamily | SSF47323 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| Superfamily | SSF47616 | Glutathione S-transferase, C-terminal domain superfamily |
| Superfamily | SSF52374 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 29 | 20 | 33 |
| disruptive | 25 | 67 | 31 | 88 |
| missense | 25 | 63 | 31 | 72 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 54 | 39 | 30 |
| disruptive | 41 | 130 | 81 | 168 |
| missense | 38 | 105 | 62 | 83 |
PlasmoDB Total SNPs: 156
Non-coding: 106 | Synonymous: 33 | Nonsynonymous: 17 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 25583729 | Inhibition of protein synthesis and malaria parasite development by drugtargeting of methionyl-tRNA synthetases. | Hussain T, Yogavel M, Sharma A | 10.1128/AAC.02220-13 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |