PF3D7_1035000

Genome location: Pf3D7_10_v3:1,386,295..1,390,034(-)

Genome classification: Core

Function and Localization

Product Description: U2 snRNA/tRNA pseudouridine synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.4.99.12 (tRNA pseudouridine(38-40) synthase);5.4.99.27 (tRNA pseudouridine(13) synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0009982	pseudouridine synthase activity
Process	GO:1990481	mRNA pseudouridine synthesis
Process	GO:0001522	pseudouridine synthesis
Process	GO:0031120	snRNA pseudouridine synthesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.27	0.87
Trophozoite	expressed	0.35	0.81
Schizont	possibly expressed	0.29	0.83
Gametocyte	expressed	0.53	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1035000

Resistome Missense Mutations: L409M

Resistome Compounds with Missense Mutations: MMV008434

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.08 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0518800)

Relative Growth Rate: 0.96 ± 0.07
Confidence: 6.66

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0518800)

Modification: Disrupted | RMgm-2027

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJ59

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.4.99.12	tRNA pseudouridine38-40 synthase	tRNApoly(rA)npoly(rC)npoly(rG)npoly(rI)npoly(rU)niodoacetateCalf thymus DNADithionitrobenzoate5-fluorouracil tRNAPhep-chloromercuribenzoatefluorouracil-substituted tRNA
5.4.99.27	tRNA pseudouridine13 synthase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101009

Most Similar Human Ortholog: Q96PZ0

TM-align score: 0.65 | RMSD: 4.34

Seq Identity: 0.28 | Length: 492 / 993

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000091127	pseudouridine synthase 7

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	31	21	21	38
disruptive	47	88	44	110
missense	43	78	42	97

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	33	45	32	44
disruptive	87	164	88	205
missense	57	143	78	146

PlasmoDB Total SNPs: 160

Non-coding: 41 | Synonymous: 71 | Nonsynonymous: 48 | Stop Codon: 0

Protein Information

Protein Length: 993 | Molecular Weight (kDa): 116.719

UniProt ID(s): Q8IJ59

PDB ID(s): None

Isoelectric Point: 8.6

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001656	Pseudouridine synthase, TruD
InterPro	IPR020103	Pseudouridine synthase, catalytic domain superfamily
InterPro	IPR020119	Pseudouridine synthase TruD, conserved site
PFam	PF01142	Pseudouridine synthase, TruD
Superfamily	SSF55120	Pseudouridine synthase, catalytic domain superfamily