Genome location: Pf3D7_10_v3:1,403,807..1,406,234(+)
Genome classification: Core
Product Description: merozoite surface protein 3
SignalP Peptide: MKSFINITLSLFLLHLYIYINNVAS
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0020037 | heme binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0044877 | protein-containing complex binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.51 | 0.83 |
| Trophozoite | possibly expressed | 0.41 | 0.78 |
| Schizont | expressed | 2.61 | 0.14 |
| Gametocyte | not expressed | 0.08 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1035400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.321 | MFS: -2.753 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ55
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 2 | 1 | 3 |
| disruptive | 52 | 59 | 11 | 61 |
| missense | 29 | 22 | 5 | 25 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 5 | 2 | 6 |
| disruptive | 83 | 78 | 37 | 79 |
| missense | 40 | 30 | 16 | 31 |
PlasmoDB Total SNPs: 217
Non-coding: 34 | Synonymous: 64 | Nonsynonymous: 119 | Stop Codon: 0
Protein Length: 354 | Molecular Weight (kDa): 40.12
UniProt ID(s): A0A4P8JE26, A0A4P8JE37, A0A4P8JE46, A0A4P8JE51, A0A4P8JE60, A0A4P8JE66, Q0KGB8, Q0KGC5, Q0KGE3, Q0KGE4, Q0KGG7, Q0KGG9, Q0KGH0, Q25705, Q25706, Q25995, Q26019, Q8IJ55, Q95PI5, Q9NFV9, Q9U6C4, V5NRL1, V5NS32
PDB ID(s): None
Isoelectric Point: 4.36
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR010784 | Merozoite surface protein-type |
| PFam | PF07133 | Merozoite surface protein-type |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11952894 | Truncation of merozoite surface protein 3 disrupts its trafficking and that ofacidic-basic repeat protein to the surface of Plasmodium falciparum merozoites. | Mills KE, Pearce JA, Crabb BS, Cowman AF | 10.1046/j.1365-2958.2002.02834.x |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17191173 | Plasmodium falciparum merozoite surface protein 3 is a target of allele-specificimmunity and alleles are maintained by natural selection. | Polley SD, Tetteh KK, ..., Conway DJ | 10.1086/509806 |
| 19404387 | A conserved multi-gene family induces cross-reactive antibodies effective indefense against Plasmodium falciparum. | Singh S, Soe S, ..., Druilhe P | 10.1371/journal.pone.0005410 |
| 19933832 | Plasmodium falciparum merozoite surface protein 1 (MSP-1)-MSP-3 chimeric protein:immunogenicity determined with human-compatible adjuvants and induction ofprotective immune response. | Mazumdar S, Mukherjee P, ..., Chauhan VS | 10.1128/IAI.00427-09 |
| 21292899 | Genetic diversity of polymorphic vaccine candidate antigens (apical membraneantigen-1, merozoite surface protein-3, and erythrocyte binding antigen-175) inPlasmodium falciparum isolates from western and central Africa. | Soulama I, Bigoga JD, ..., Sirima SB | 10.4269/ajtmh.2011.10-0365 |
| 24362023 | Plasmodium falciparum merozoite surface protein 3: oligomerization,self-assembly, and heme complex formation. | Imam M, Singh S, Kaushik NK, Chauhan VS | 10.1074/jbc.M113.520239 |
| 24635878 | Polymorphic patterns of the merozoite surface protein-3beta in Korean isolates ofPlasmodium vivax. | Kang JM, Ju HL, ..., Na BK | 10.1186/1475-2875-13-104 |
| 26823464 | Multiple Plasmodium falciparum Merozoite Surface Protein 1 Complexes MediateMerozoite Binding to Human Erythrocytes. | Lin CS, Uboldi AD, ..., Cowman AF | 10.1074/jbc.M115.698282 |
| 29760216 | Plasmodium falciparum M SP3 Exists in a Complex on the Merozoite Surface and Generates Antibody Response during Natural Infection | Deshmukh A, Chourasia BK, ..., Malhotra P | 10.1128/IAI.00067-18 |
| 37624328 | High Prevalence of Polyclonal Plasmodium falciparum Infections and Associationwith Poor IgG Antibody Responses in a Hyper-Endemic Area in Cameroon | Biabi MFAB, Fogang B, ..., Ayong L | 10.3390/tropicalmed8080390 |
| 35293561 | Plasmodium falciparum merozoite surface protein 3 as a vaccine candidate: a briefreview | Alves KCS, Guimaraes JM, Almeida MEM, Mariuba LAM | 10.1590/S1678-9946202264023 |
| 37515848 | Are high avidity antibodies to Plasmodium falciparum antigens preferentiallytransferred across the placenta of premature and term babies? | Kayatani AKK, Bobbili N, ..., Taylor DW | 10.1016/j.placenta.2023.07.011 |
| 35720297 | GMZ2 Vaccine-Induced Antibody Responses, Naturally Acquired Immunity and theIncidence of Malaria in Burkinabe Children | Dassah S, Adu B, ..., Theisen M | 10.3389/fimmu.2022.899223 |
| 36396703 | The first complete genome of the simian malaria parasite Plasmodium brasilianum | Bajic M, Ravishankar S, ..., Talundzic E | 10.1038/s41598-022-20706-6 |
| 35355994 | Plasmodium falciparum-Specific Memory B-Cell and Antibody Responses AreAssociated With Immunity in Children Living in an Endemic Area of Kenya | Jahnmatz P, Nyabundi D, ..., Farnert A | 10.3389/fimmu.2022.799306 |
| 36846776 | Retrospective analysis of Plasmodium vivax genomes from a pre-elimination Chinainland population in the 2010s | Liu Y, Zhang T, ..., Chen JH | 10.3389/fmicb.2023.1071689 |
| 35141172 | Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivaxPatients | Aparici-Herraiz I, Gualdron-Lopez M, ..., Del Portillo HA | 10.3389/fcimb.2021.811390 |
| 35411125 | Antibody Responses Against Plasmodium falciparum MSP3 Protein During NaturalMalaria Infection in Individuals Living in Malaria-Endemic Regions of India | Quadiri A, Kori L, Singh SK, Anvikar AR | 10.1007/s40011-021-01330-2 |
| 36403045 | Characterization of the primary antibody response to Plasmodium falciparumantigens in infants living in a malaria-endemic area | Tassi Yunga S, Siriwardhana C, ..., Taylor DW | 10.1186/s12936-022-04360-x |
| 36692923 | The Effects of HIV Infection on the Immune Response to Malaria Among PregnantWomen in Kumba, Southwest Cameroon: Protocol for a Cross-sectional Study | Obase BN, Francis Z, ..., Nsagha DS | 10.2196/38213 |
| 36499205 | Fc Gamma Receptor IIIB NA1/NA2/SH Polymorphisms Are Associated with MalariaSusceptibility and Antibody Levels to P. falciparum Merozoite Antigens inBeninese Children | Fall AKDJ, Courtin D, ..., Dechavanne C | 10.3390/ijms232314882 |
| 37501921 | Plasmodium falciparum infection and naturally acquired immunity to malariaantigens among Ghanaian children in northern Ghana | Seidu Z, Lamptey H, ..., Ofori MF | 10.1016/j.parepi.2023.e00317 |
| 35697275 | Cyclic constrained immunoreactive peptides from crucial P. falciparum proteins:potential implications in malaria diagnostics | Vashisht K, Srivastava S, ..., Pandey KC | 10.1016/j.trsl.2022.06.008 |
| 36851323 | B-Cell Epitope Mapping of the Plasmodium falciparum Malaria Vaccine CandidateGMZ2.6c in a Naturally Exposed Population of the Brazilian Amazon | Baptista BO, Souza ABL, ..., Pratt-Riccio LR | 10.3390/vaccines11020446 |
| 35637187 | Stochastic expression of invasion genes in Plasmodium falciparum schizonts | Tripathi J, Zhu L, ..., Bozdech Z | 10.1038/s41467-022-30605-z |
| 37037443 | Serological Markers of Exposure to Plasmodium falciparum and Plasmodium vivaxInfection in Southwestern Ethiopia | Jeang B, Lee MC, ..., Dent A | 10.4269/ajtmh.22-0645 |