About Gene List

PF3D7_1036300 (DBLMSP2)

Genome location: Pf3D7_10_v3:1,432,498..1,434,786(+)

Genome classification: Core

Function and Localization

Product Description: duffy binding-like merozoite surface protein 2

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0009986 cell surface
Function GO:0001791 IgM binding
Function GO:0046812 host cell surface binding
Function GO:0005515 protein binding
Process GO:0042493 response to drug
Process GO:0009410 response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.01 0.99
Trophozoite not expressed 0.01 1.00
Schizont expressed 0.09 0.96
Gametocyte not expressed 0.01 1.00

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1036300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.844 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJ45

"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=0.99) with 5MZA (Global RMSD=5.75)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_160723

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 35 7 9 19
disruptive 137 99 34 115
missense 119 61 26 94

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 36 17 14 24
disruptive 165 139 60 114
missense 137 90 51 84

PlasmoDB Total SNPs: 289

Non-coding: 0 | Synonymous: 80 | Nonsynonymous: 207 | Stop Codon: 2

Protein Information

Protein Length: 762 | Molecular Weight (kDa): 87.573

UniProt ID(s): D9HN87, D9HN89, D9HN99, D9HNA1, D9HNB5, D9HNB6, D9HNC4, D9HND2, D9HNE4, D9HNE5, Q8IJ45

PDB ID(s): 3VUU, 3VUV

Isoelectric Point: 4.43

Protein Domain Annotations:

Source Family ID Description
InterPro IPR008602 Duffy-antigen binding
InterPro IPR010784 Merozoite surface protein-type
PFam PF05424 Duffy-antigen binding
PFam PF07133 Merozoite surface protein-type
Superfamily SSF140924 N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
21533027 Identification and functional validation of the novel antimalarial resistancelocus PF10_0355 in Plasmodium falciparum. Van Tyne D, Park DJ, ..., Sabeti PC 10.1371/journal.pgen.1001383
22843685 Insights into Duffy binding-like domains through the crystal structure andfunction of the merozoite surface protein MSPDBL2 from Plasmodium falciparum. Hodder AN, Czabotar PE, ..., Cowman AF 10.1074/jbc.M112.350504
23587962 Modulation of PF10_0355 (MSPDBL2) alters Plasmodium falciparum response toantimalarial drugs. Van Tyne D, Uboldi AD, ..., Wirth DF 10.1128/AAC.02574-12
26823464 Multiple Plasmodium falciparum Merozoite Surface Protein 1 Complexes MediateMerozoite Binding to Human Erythrocytes. Lin CS, Uboldi AD, ..., Cowman AF 10.1074/jbc.M115.698282
27226583 Binding of Plasmodium falciparum Merozoite Surface Proteins DBLMSP and DBLMSP2 toHuman Immunoglobulin M Is Conserved among Broadly Diverged Sequence Variants. Crosnier C, Iqbal Z, ..., Wright GJ 10.1074/jbc.M116.722074
30526479 Schizont transcriptome variation among clinical isolates and laboratory-adaptedclones of the malaria parasite Plasmodium falciparum Tarr SJ, Diaz-Ingelmo O, ..., Conway DJ 10.1186/s12864-018-5257-x
37156765 Plasmodium falciparum has evolved multiple mechanisms to hijack humanimmunoglobulin M Ji C, Shen H, ..., Xiao J 10.1038/s41467-023-38320-z