About Gene List

PF3D7_1037000 (REV3)

Genome location: Pf3D7_10_v3:1,461,927..1,469,867(+)

Genome classification: Core

Function and Localization

Product Description: DNA polymerase zeta catalytic subunit, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.7 (DNA-directed DNA polymerase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus
Component GO:0016035 zeta DNA polymerase complex
Function GO:0003887 DNA-directed DNA polymerase activity
Process GO:0000724 double-strand break repair via homologous recombination
Process GO:0042276 error-prone translesion synthesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.36 0.83
Trophozoite possibly expressed 0.74 0.60
Schizont possibly expressed 0.31 0.82
Gametocyte not expressed 0.42 0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1037000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.782 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZYZ7

"Best" AlphaFill ligand hit: DG3 (2'-3'-dideoxyguanosine-5'-triphosphate, Local RMSD=0.47) with 3K5M (Global RMSD=4.01)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.7.7 DNA-directed DNA polymerase KClEDTANH4+dATPNH4ClddTTPMK886UracilalliinsucroseethanolAllicin...

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P28340 DNA polymerase delta catalytic subunit OrthoDB Combivir

Orthology Information

Ortholog Group (OrthoMCL): OG6_533491

No human ortholog(s)

Protein Information

Protein Length: 2226 | Molecular Weight (kDa): 265.947

UniProt ID(s): A0A143ZYZ7

PDB ID(s): None

Isoelectric Point: 8.14

Protein Domain Annotations:

Source Family ID Description
InterPro IPR006134 DNA-directed DNA polymerase, family B, multifunctional domain
InterPro IPR006172 DNA-directed DNA polymerase, family B
InterPro IPR012337 Ribonuclease H-like superfamily
InterPro IPR017964 DNA-directed DNA polymerase, family B, conserved site
InterPro IPR030559 DNA polymerase zeta catalytic subunit
InterPro IPR043502 DNA/RNA polymerase superfamily
PFam PF00136 DNA-directed DNA polymerase, family B, multifunctional domain
Superfamily SSF49562 Ribonuclease H-like superfamily
Superfamily SSF53098 DNA/RNA polymerase superfamily
Superfamily SSF56672

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 27 97 56 92
disruptive 111 292 153 294
missense 63 251 134 236

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 53 157 65 98
disruptive 175 511 222 412
missense 108 414 171 311

PlasmoDB Total SNPs: 335

Non-coding: 57 | Synonymous: 141 | Nonsynonymous: 134 | Stop Codon: 3

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
36140745 DNA Polymerase zeta without the C-Terminus of Catalytic Subunit Rev3 RetainsCharacteristic Activity, but Alters Mutation Specificity of Ultraviolet Radiationin Yeast Siebler HM, Cui J, Hill SE, Pavlov YI 10.3390/genes13091576