Genome location: Pf3D7_10_v3:1,469,672..1,472,655(-)
Genome classification: Core
Product Description: pyruvate kinase 2
SignalP Peptide: MNLIHICLFIIVIKCYVNC
# Transmembrane Domains: 0
EC Numbers: 2.7.1.40 (Pyruvate kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Function | GO:0004743 | pyruvate kinase activity |
| Process | GO:0006096 | glycolytic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.21 | 0.90 |
| Trophozoite | expressed | 0.89 | 0.54 |
| Schizont | expressed | 0.10 | 0.94 |
| Gametocyte | expressed | 0.40 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1037100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.01 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0520000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ37
"Best" AlphaFill ligand hit: 144 (tris-hydroxymethyl-methyl-ammonium, Local RMSD=0.14) with 6V74 (Global RMSD=1.35)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.40 | pyruvate kinase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 40 | 16 | 36 |
| disruptive | 12 | 44 | 34 | 80 |
| missense | 10 | 41 | 32 | 75 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 67 | 17 | 38 |
| disruptive | 19 | 118 | 63 | 109 |
| missense | 14 | 101 | 55 | 81 |
PlasmoDB Total SNPs: 105
Non-coding: 66 | Synonymous: 22 | Nonsynonymous: 17 | Stop Codon: 0
Protein Length: 745 | Molecular Weight (kDa): 86.685
UniProt ID(s): Q8IJ37
PDB ID(s): None
Isoelectric Point: 8.01
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001697 | Pyruvate kinase |
| InterPro | IPR011037 | Pyruvate kinase-like, insert domain superfamily |
| InterPro | IPR015793 | Pyruvate kinase, barrel |
| InterPro | IPR015795 | Pyruvate kinase, C-terminal |
| InterPro | IPR015813 | Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily |
| InterPro | IPR018209 | Pyruvate kinase, active site |
| InterPro | IPR036918 | Pyruvate kinase, C-terminal domain superfamily |
| PFam | PF00224 | Pyruvate kinase, barrel |
| PFam | PF02887 | Pyruvate kinase, C-terminal |
| Superfamily | SSF50800 | Pyruvate kinase-like, insert domain superfamily |
| Superfamily | SSF51621 | Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily |
| Superfamily | SSF52935 | Pyruvate kinase, C-terminal domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 19015045 | Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to theapicoplast. | Maeda T, Saito T, ..., Asai T | 10.1016/j.parint.2008.10.005 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 32815516 | The N TP generating activity of pyruvate kinase I I is critical for apicoplastmaintenance in Plasmodium falciparum | Swift RP, Rajaram K, ..., Prigge ST | 10.7554/eLife.50807 |