PF3D7_1038900

Genome location: Pf3D7_10_v3:1,561,978..1,564,670(+)

Genome classification: Core

Function and Localization

Product Description: esterase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.-.- (Acting on ester bonds.);3.1.1.5 (Lysophospholipase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016020	membrane
Component	GO:0005739	mitochondrion
Function	GO:0016787	hydrolase activity
Function	GO:0016788	hydrolase activity, acting on ester bonds
Function	GO:0016298	lipase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.01	1.00
Trophozoite	expressed	0.05	0.97
Schizont	expressed	0.02	0.98
Gametocyte	expressed	0.03	0.98

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1038900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.123 | MFS: -3.112 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1220300)

Relative Growth Rate: 1.09 ± 0.18
Confidence: 4.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IJ22

"Best" AlphaFill ligand hit: COA (coenzyme a, Local RMSD=11.33) with 5FRD (Global RMSD=14.07)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.-.-	Ex: 5-phospho-D-xylono-1,4-lactonase	paraoxonatropineethylene terephthalatediisopropyl fluorophosphonatebis(2-hydroxyethyl) terephthalic acidphosphoric acid-bis-4-nitrophenyl ester
3.1.1.5	lysophospholipase	NEMSDSEDTAPCMBEGTAPMSFDMSOCaCl2FeCl3xyleneethanolsulfite...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100231

Most Similar Human Ortholog: A0A3B3ITT3

TM-align score: 0.86 | RMSD: 1.90

Seq Identity: 0.19 | Length: 242 / 359

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000074416	monoglyceride lipase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	8	6	10
disruptive	8	5	2	22
missense	7	5	2	20

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	15	9	17
disruptive	11	19	18	74
missense	10	15	15	59

PlasmoDB Total SNPs: 74

Non-coding: 61 | Synonymous: 7 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 359 | Molecular Weight (kDa): 41.77

UniProt ID(s): Q8IJ22

PDB ID(s): None

Isoelectric Point: 6.83

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006494	Plasmodium subtelomeric PST-A
InterPro	IPR022742	Serine aminopeptidase, S33
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF12146	Serine aminopeptidase, S33
Superfamily	SSF53474	Alpha/Beta hydrolase fold

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21