Genome location: Pf3D7_10_v3:1,567,784..1,572,695(+)
Genome classification: Core
Product Description: serine/threonine protein kinase, FIKK family
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0044003 | modulation by symbiont of host process |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.63 | 0.71 |
| Trophozoite | expressed | 0.46 | 0.79 |
| Schizont | possibly expressed | 0.14 | 0.92 |
| Gametocyte | not expressed | 0.06 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1039000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.996 | MFS: -0.296 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1225000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IJ21
"Best" AlphaFill ligand hit: NIO (nicotinic acid, Local RMSD=0.57) with 5Y9M (Global RMSD=11.13)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 25 | 21 | 40 |
| disruptive | 49 | 95 | 47 | 129 |
| missense | 44 | 89 | 45 | 116 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 53 | 22 | 51 |
| disruptive | 83 | 179 | 100 | 202 |
| missense | 69 | 147 | 90 | 143 |
PlasmoDB Total SNPs: 229
Non-coding: 141 | Synonymous: 44 | Nonsynonymous: 44 | Stop Codon: 0
Protein Length: 913 | Molecular Weight (kDa): 107.625
UniProt ID(s): Q8IJ21
PDB ID(s): None
Isoelectric Point: 4.92
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 31650539 | Identification of essential exported Plasmodium falciparum protein kinases inmalaria-infected red blood cells. | Siddiqui G, Proellochs NI, Cooke BM | 10.1111/bjh.16219 |
| 32284562 | An exported kinase family mediates species-specific erythrocyte remodelling andvirulence in human malaria. | Davies H, Belda H, ..., Treeck M | 10.1038/s41564-020-0702-4 |