Genome location: Pf3D7_11_v3:32,666..42,386(-)
Genome classification: SubtelomericHypervariable
Product Description: erythrocyte membrane protein 1, PfEMP1
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0020030 | infected host cell surface knob |
| Function | GO:0050839 | cell adhesion molecule binding |
| Function | GO:0046789 | host cell surface receptor binding |
| Process | GO:0020033 | antigenic variation |
| Process | GO:0098609 | cell-cell adhesion |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
| Process | GO:0020013 | modulation by symbiont of host erythrocyte aggregation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.01 | 0.99 |
| Trophozoite | expressed | 0.01 | 1.00 |
| Schizont | expressed | 0.01 | 1.00 |
| Gametocyte | expressed | 0.02 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1100200
Resistome Missense Mutations: T295S, P297A, K892T, D894E, K895I, V898E, D899N, E900D, D901H, G902A, M1240T, M1240I, K2762R, K2762N, N2882S, I2897M, N2900T
Resistome Compounds with Missense Mutations: AN13762, Cipargamin/KAE609/NITD609
Resistome # Samples with Disruptive Mutations: 3 (3 missense, 3 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.315 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 2931
Non-coding: 297 | Synonymous: 669 | Nonsynonymous: 1962 | Stop Codon: 3
Protein Length: 2994 | Molecular Weight (kDa): 345.69
UniProt ID(s): A0A0U1XT24, A0A191VZ32, A0A191VZ39, A0A191VZ47, A0A191VZ53, A0A191VZ76, A0A191VZC3, A0A191VZC9, A0A5P1A013, A0A5P1A0C0, A0A5P1A0G2, A0A5P1A0K0, A0A5P1A0L1, A0A5P1A0L9, A0A5P1A0M1, A0A5P1A0R8, A0A5P1A0S8, A0A5P1A103, A0A5P1A134, A0A5P1A161, A0A5P1A1C6, A0A5P1A1H5, A0A5P1A1J4, A0A5P1A229, A0A5P1A244, A0A5P1A294, A0A5P1A2F4, A0A5P1A2J7, A0A5P1A2K0, A1KQT3, I2CIW6, I2CIX1, I2CIX3, Q6UDX0, Q8IIZ4
PDB ID(s): None
Isoelectric Point: 5.51
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004258 | Duffy-binding-like domain |
| InterPro | IPR008602 | Duffy-antigen binding |
| InterPro | IPR029210 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| InterPro | IPR029211 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| InterPro | IPR041480 | Cysteine-rich interdomain region 1 gamma |
| PFam | PF03011 | Duffy-binding-like domain |
| PFam | PF05424 | Duffy-antigen binding |
| PFam | PF15445 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| PFam | PF15447 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| PFam | PF18562 | Cysteine-rich interdomain region 1 gamma |
| Superfamily | SSF140924 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9230440 | P. falciparum rosetting mediated by a parasite-variant erythrocyte membraneprotein and complement-receptor 1. | Rowe JA, Moulds JM, Newbold CI, Miller LH | 10.1038/40888 |
| 9291676 | Knob proteins in falciparum malaria. | Sharma YD | https://pubmed.ncbi.nlm.nih.gov/9291676/ |
| 9786187 | A recombinant peptide based on PfEMP-1 blocks and reverses adhesion ofmalaria-infected red blood cells to CD36 under flow. | Cooke BM, Nicoll CL, Baruch DI, Coppel RL | 10.1046/j.1365-2958.1998.01040.x |
| 11316367 | Variant antigens of Plasmodium falciparum encoded by the var multigenic familyare multifunctional macromolecules. | Nogueira PA, Wunderlich G, Pereira da Silva LH | 10.1016/s0923-2508(01)01184-6 |
| 11420100 | Molecules on the surface of the Plasmodium falciparum infected erythrocyte andtheir role in malaria pathogenesis and immune evasion. | Craig A, Scherf A | https://pubmed.ncbi.nlm.nih.gov/11420100/ |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18785843 | Antigenic variation in Plasmodium falciparum. | Scherf A, Lopez-Rubio JJ, Riviere L | 10.1146/annurev.micro.61.080706.093134 |