PF3D7_1102400 (PPCS)

Genome location: Pf3D7_11_v3:114,236..118,021(-)

Genome classification: Core

Function and Localization

Product Description: phosphopantothenate--cysteine ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.3.2.5 (Phosphopantothenate--cysteine ligase (CTP))

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0004632	phosphopantothenate--cysteine ligase activity
Process	GO:0015937	coenzyme A biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.19	0.90
Trophozoite	expressed	0.32	0.80
Schizont	expressed	0.33	0.79
Gametocyte	expressed	0.21	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1102400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.997 | MFS: -0.546 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0613600)

Relative Growth Rate: 0.96 ± 0.11
Confidence: 5.72

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_0616300)

Modification: Disrupted | RMgm-4182

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIX6

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.19) with 7EDZ (Global RMSD=3.42)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.3.2.5	phosphopantothenate-cysteine ligase (CTP)	ATPAMPGTPCTPUTPKClCMPEDTAL-Cysphosphate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102247

Most Similar Human Ortholog: Q9HAB8

TM-align score: 0.85 | RMSD: 2.38

Seq Identity: 0.30 | Length: 290 / 660

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000127125	phosphopantothenoylcysteine synthetase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	12	23	11	22
disruptive	47	100	51	100
missense	37	83	45	88

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	19	33	18	34
disruptive	79	180	73	153
missense	61	139	59	112

PlasmoDB Total SNPs: 198

Non-coding: 132 | Synonymous: 23 | Nonsynonymous: 43 | Stop Codon: 0

Protein Information

Protein Length: 660 | Molecular Weight (kDa): 78.535

UniProt ID(s): Q8IIX6

PDB ID(s): None

Isoelectric Point: 7.04

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR035929	CoaB-like superfamily
PFam	N/A	N/A
Superfamily	SSF102645	CoaB-like superfamily