PF3D7_1103600 (ALP2a)

Genome location: Pf3D7_11_v3:156,062..158,420(+)

Genome classification: Core

Function and Localization

Product Description: actin-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0035267	NuA4 histone acetyltransferase complex
Component	GO:0016514	SWI/SNF complex
Component	GO:0005634	nucleus
Function	GO:0003682	chromatin binding
Process	GO:0043044	ATP-dependent chromatin remodeling
Process	GO:0043967	histone H4 acetylation
Process	GO:0006357	regulation of transcription by RNA polymerase II

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.13	0.93
Trophozoite	expressed	0.25	0.84
Schizont	expressed	0.03	0.98
Gametocyte	expressed	0.12	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1103600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.282 | MFS: -2.74 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0943300)

Relative Growth Rate: 0.43 ± 0.23
Confidence: 4.30

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0943300)

Modification: Disrupted | RMgm-2692

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIW6

"Best" AlphaFill ligand hit: SPD (n-(2-amino-propyl)-1,4-diaminobutane, Local RMSD=0.30) with 2HMP (Global RMSD=1.40)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_171465

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	23	11	21
disruptive	8	27	8	42
missense	6	25	8	34

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	43	20	26
disruptive	13	56	31	88
missense	9	45	26	56

PlasmoDB Total SNPs: 143

Non-coding: 118 | Synonymous: 22 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 499 | Molecular Weight (kDa): 57.97

UniProt ID(s): Q8IIW6

PDB ID(s): None

Isoelectric Point: 6.68

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004000	Actin family
InterPro	IPR043129	ATPase, nucleotide binding domain
PFam	PF00022	Actin family
Superfamily	SSF53067	ATPase, nucleotide binding domain

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108