Genome location: Pf3D7_11_v3:159,527..161,502(+)
Genome classification: Core
Product Description: casein kinase II beta chain
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Component | GO:0005956 | protein kinase CK2 complex |
| Function | GO:0019887 | protein kinase regulator activity |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.24 | 0.88 |
| Trophozoite | expressed | 0.47 | 0.71 |
| Schizont | expressed | 0.08 | 0.94 |
| Gametocyte | expressed | 0.22 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1103700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -2.886 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0943200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIW5
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P67870 | Casein kinase II subunit beta | HOGENOM, OrthoDB | US9303033, N47, Table 58A, Compound 11US9303033, Q47, Table 58A, Compound 14BDBM220085US9303033, C1, Table 2A, Compound 1... |
Ortholog Group (OrthoMCL): OG6_100687
Most Similar Human Ortholog: P67870
TM-align score: 0.80 | RMSD: 2.27
Seq Identity: 0.37 | Length: 188 / 245
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000204435 | casein kinase 2 beta |
| ENSG00000230700 | casein kinase 2 beta |
| ENSG00000232960 | casein kinase 2 beta |
Protein Length: 245 | Molecular Weight (kDa): 28.365
UniProt ID(s): Q8IIW5
PDB ID(s): None
Isoelectric Point: 7.34
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000704 | Casein kinase II, regulatory subunit |
| InterPro | IPR035991 | Casein kinase II subunit beta-like |
| PFam | PF01214 | Casein kinase II, regulatory subunit |
| Superfamily | SSF57798 | Casein kinase II subunit beta-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 18 | 11 | 13 |
| disruptive | 2 | 6 | 4 | 20 |
| missense | 2 | 4 | 3 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 37 | 8 | 11 |
| disruptive | 3 | 34 | 23 | 59 |
| missense | 3 | 23 | 16 | 41 |
PlasmoDB Total SNPs: 79
Non-coding: 69 | Synonymous: 10 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 19114502 | Functional analysis of protein kinase CK2 of the human malaria parasitePlasmodium falciparum. | Holland Z, Prudent R, ..., Doerig C | 10.1128/EC.00334-08 |
| 22293287 | Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assemblypathway. | Dastidar EG, Dayer G, ..., Doerig C | 10.1186/1741-7007-10-5 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 31723180 | Mapping and functional analysis of heterochromatin protein 1 phosphorylation inthe malaria parasite Plasmodium falciparum. | Bui HTN, Niederwieser I, ..., Voss TS | 10.1038/s41598-019-53325-9 |
| 29197418 | Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9 | Kuang D, Qiao J, ..., Dai X | 10.1186/s13071-017-2539-0 |