Product Description: phenylalanine--tRNA ligase beta subunit
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.20 (Phenylalanine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005622 | intracellular |
| Component | GO:0005634 | nucleus |
| Component | GO:0009328 | phenylalanine-tRNA ligase complex |
| Function | GO:0004826 | phenylalanine-tRNA ligase activity |
| Process | GO:0006432 | phenylalanyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.52 | 0.75 |
| Trophozoite | expressed | 1.14 | 0.43 |
| Schizont | expressed | 0.20 | 0.87 |
| Gametocyte | expressed | 0.30 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1104000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.13 | MFS: -2.954 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0942900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_0945400)
Modification: Tagged | RMgm-1558
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIW2
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.20 | phenylalanine-tRNA ligase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101233
Most Similar Human Ortholog: Q9NSD9
TM-align score: 0.78 | RMSD: 4.51
Seq Identity: 0.35 | Length: 565 / 623
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000116120 | phenylalanyl-tRNA synthetase subunit beta |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 37 | 20 | 50 |
| disruptive | 3 | 75 | 36 | 80 |
| missense | 3 | 75 | 36 | 75 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 62 | 28 | 55 |
| disruptive | 17 | 140 | 60 | 133 |
| missense | 12 | 135 | 58 | 107 |
PlasmoDB Total SNPs: 79
Non-coding: 49 | Synonymous: 17 | Nonsynonymous: 13 | Stop Codon: 0
Protein Length: 623 | Molecular Weight (kDa): 72.502
UniProt ID(s): Q8IIW2
PDB ID(s): None
Isoelectric Point: 5.57
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004531 | Phenylalanyl-tRNA synthetase, class IIc, beta subunit, archae/euk cytosolic |
| InterPro | IPR005146 | B3/B4 tRNA-binding domain |
| InterPro | IPR005147 | tRNA synthetase, B5-domain |
| InterPro | IPR009061 | Putative DNA-binding domain superfamily |
| InterPro | IPR040659 | Phenylalanine--tRNA ligase beta subunit, B1 domain |
| InterPro | IPR041616 | Phenylalanyl tRNA synthetase beta chain, core domain |
| PFam | PF03483 | B3/B4 tRNA-binding domain |
| PFam | PF03484 | tRNA synthetase, B5-domain |
| PFam | PF17759 | Phenylalanyl tRNA synthetase beta chain, core domain |
| PFam | PF18262 | Phenylalanine--tRNA ligase beta subunit, B1 domain |
| Superfamily | SSF46955 | Putative DNA-binding domain superfamily |
| Superfamily | SSF55681 | |
| Superfamily | SSF56037 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27503796 | Proteome mapping of Plasmodium: identification of the P. yoelii remodellome. | Siau A, Huang X, ..., Preiser PR | 10.1038/srep31055 |