Product Description: DNA-directed RNA polymerase III subunit RPC8, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005666 | RNA polymerase III complex |
| Process | GO:0006384 | transcription initiation from RNA polymerase III promoter |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | nd | nd |
| Trophozoite | expressed | nd | nd |
| Schizont | expressed | nd | nd |
| Gametocyte | expressed | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1104700.2
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.279 | MFS: -3.238 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIV5
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101831
Most Similar Human Ortholog: Q9Y535
TM-align score: 0.93 | RMSD: 1.88
Seq Identity: 0.32 | Length: 186 / 186
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100413 | RNA polymerase III subunit H |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 7 | 2 | 7 |
| disruptive | 0 | 6 | 6 | 16 |
| missense | 0 | 6 | 6 | 16 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 15 | 5 | 13 |
| disruptive | 1 | 21 | 27 | 50 |
| missense | 1 | 16 | 20 | 38 |
PlasmoDB Total SNPs: 68
Non-coding: 54 | Synonymous: 13 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 186 | Molecular Weight (kDa): 21.926
UniProt ID(s): Q8IIV5
PDB ID(s): None
Isoelectric Point: 4.99
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005576 | RNA polymerase Rpb7-like , N-terminal |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR013238 | RNA polymerase III, subunit Rpc25 |
| InterPro | IPR036898 | RNA polymerase Rpb7-like, N-terminal domain superfamily |
| PFam | PF03876 | RNA polymerase Rpb7-like , N-terminal |
| PFam | PF08292 | RNA polymerase III, subunit Rpc25 |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF88798 | RNA polymerase Rpb7-like, N-terminal domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21714024 | Crystal structure of the C17/25 subcomplex from Schizosaccharomyces pombe RNApolymerase III | Ehara H, Sekine S, Yokoyama S | 10.1002/pro.682 |
| 10645483 | [Characteristics of the cDNA of the Schizosaccharomyces pombe rpa43+ gene:structural similarity of the Rpa43 subunit of RNA-polymerase I with the Rpc25subunit of RNA-polymerase III] | Shpakovskii GV, Shematorova EK | https://pubmed.ncbi.nlm.nih.gov/10645483/ |
| 21983101 | Rpa43 and its partners in the yeast RNA polymerase I transcription complex | Beckouet F, Mariotte-Labarre S, ..., Thuriaux P | 10.1016/j.febslet.2011.09.011 |
| 12482973 | An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue ofmammalian CGRP-RCP | Siaut M, Zaros C, ..., Conesa C | 10.1128/MCB.23.1.195-205.2003 |