PF3D7_1105600 (PTEX88)

Genome location: Pf3D7_11_v3:240,616..245,317(-)

Genome classification: Core

Function and Localization

Product Description: translocon component PTEX88

SignalP Peptide: MIYQYIFVLCILLVHVGQC

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0097619	PTEX complex
Component	GO:0020026	merozoite dense granule
Component	GO:0020003	symbiont-containing vacuole
Component	GO:0020005	symbiont-containing vacuole membrane
Function	GO:0003723	RNA binding
Function	GO:0005515	protein binding
Process	GO:0044053	translocation of peptides or proteins into host cell cytoplasm

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.62	0.35
Trophozoite	expressed	0.57	0.68
Schizont	expressed	0.25	0.85
Gametocyte	not expressed	0.32	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1105600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.132 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0941300)

Relative Growth Rate: 0.34 ± 0.16
Confidence: 5.05

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0941300)

Modification: Disrupted | RMgm-2681

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIU7

"Best" AlphaFill ligand hit: UDP (uridine-5'-diphosphate, Local RMSD=0.38) with 4X6L (Global RMSD=22.61)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532732

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	65	29	67
disruptive	23	74	51	95
missense	23	72	48	89

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	117	41	68
disruptive	35	169	73	240
missense	31	150	63	156

PlasmoDB Total SNPs: 234

Non-coding: 181 | Synonymous: 25 | Nonsynonymous: 28 | Stop Codon: 0

Protein Information

Protein Length: 777 | Molecular Weight (kDa): 90.787

UniProt ID(s): Q8IIU7

PDB ID(s): None

Isoelectric Point: 8.01

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
23869529	The Plasmodium translocon of exported proteins (PTEX) component thioredoxin-2 isimportant for maintaining normal blood-stage growth.	Matthews K, Kalanon M, ..., de Koning-Ward TF	10.1111/mmi.12334
27168322	Stable Translocation Intermediates Jam Global Protein Export in Plasmodiumfalciparum Parasites and Link the PTEX Component EXP2 with TranslocationActivity.	Mesen-Ramirez P, Reinsch F, ..., Spielmann T	10.1371/journal.ppat.1005618
29637707	The malaria PTEX component PTEX88 interacts most closely with HSP101 at thehost-parasite interface.	Chisholm SA, Kalanon M, ..., de Koning-Ward TF	10.1111/febs.14463
33906926	Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH	Sinha A, Baumgarten S, ..., Scherf A	10.1128/mBio.00661-21
32521667	Crossing the Vacuolar Rubicon: Structural Insights into Effector ProteinTrafficking in Apicomplexan Parasites	Egea PF	10.3390/microorganisms8060865
24076174	Two putative protein export regulators promote Plasmodium blood stage developmentin vivo	Matz JM, Matuschewski K, Kooij TW	10.1016/j.molbiopara.2013.09.003
26219962	The Plasmodium berghei translocon of exported proteins reveals spatiotemporaldynamics of tubular extensions	Matz JM, Goosmann C, ..., Kooij TW	10.1038/srep12532
26886275	Contrasting Inducible Knockdown of the Auxiliary PTEX Component PTEX88 in P.falciparum and P. berghei Unmasks a Role in Parasite Virulence	Chisholm SA, McHugh E, ..., de Koning-Ward TF	10.1371/journal.pone.0149296
19536257	A newly discovered protein export machine in malaria parasites	de Koning-Ward TF, Gilson PR, ..., Crabb BS	10.1038/nature08104
27865834	Expression and characterization of the Plasmodium translocon of the exportedproteins component EXP2	Hakamada K, Watanabe H, ..., Yohda M	10.1016/j.bbrc.2016.11.097
25820521	In Vivo Function of PTEX88 in Malaria Parasite Sequestration and Virulence	Matz JM, Ingmundson A, ..., Kooij TW	10.1128/EC.00276-14