Product Description: tRNA-splicing ligase RtcB, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.5.1.8 (3'-phosphate/5'-hydroxy nucleic acid ligase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0005634 | nucleus |
Component | GO:0072669 | tRNA-splicing ligase complex |
Function | GO:0003972 | RNA ligase (ATP) activity |
Process | GO:0000971 | tRNA exon ligation utilizing 2',3' cyclic phosphate of 5'-exon as source of linkage phosphate |
Process | GO:0006388 | tRNA splicing, via endonucleolytic cleavage and ligation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | expressed | N/A | N/A |
Ring | expressed | 0.28 | 0.86 |
Trophozoite | expressed | 0.23 | 0.85 |
Schizont | expressed | 0.06 | 0.96 |
Gametocyte | expressed | 0.22 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1105700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.164 | MFS: -2.833 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIU6
"Best" AlphaFill ligand hit: 5GP (guanosine-5'-monophosphate, Local RMSD=0.53) with 7P3B (Global RMSD=0.81)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
6.5.1.8 | 3'-phosphate/5'-hydroxy nucleic acid ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101445
Most Similar Human Ortholog: Q9Y3I0
TM-align score: 0.99 | RMSD: 1.07
Seq Identity: 0.64 | Length: 504 / 506
All Human Orthologs (OrthoMCL):
Gene ID | Description |
---|---|
ENSG00000100220 | RNA 2,3-cyclic phosphate and 5-OH ligase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 3 | 31 | 13 | 37 |
disruptive | 6 | 35 | 16 | 50 |
missense | 6 | 35 | 16 | 47 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 10 | 63 | 23 | 30 |
disruptive | 12 | 66 | 41 | 126 |
missense | 10 | 63 | 37 | 69 |
PlasmoDB Total SNPs: 134
Non-coding: 104 | Synonymous: 20 | Nonsynonymous: 10 | Stop Codon: 0
Protein Length: 506 | Molecular Weight (kDa): 56.321
UniProt ID(s): A0A143ZZZ9, Q8IIU6
PDB ID(s): None
Isoelectric Point: 8.29
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR001233 | RNA-splicing ligase, RtcB |
InterPro | IPR027513 | RNA-splicing ligase RtcB homologue, eukaryotic |
InterPro | IPR036025 | tRNA-splicing ligase RtcB-like superfamily |
PFam | PF01139 | RNA-splicing ligase, RtcB |
Superfamily | SSF103365 | tRNA-splicing ligase RtcB-like superfamily |
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
37935993 | Insights into the structure and function of the RNA ligase RtcB | Moncan M, Rakhsh-Khorshid H, ..., Gorman AM | 10.1007/s00018-023-05001-5 |
37648453 | Thioredoxin regulates the redox state and the activity of the human tRNA ligase complex | Jaksch D, Irnstorfer J, Kalman PF, Martinez J | 10.1261/rna.079732.123 |
35742896 | RtcB2-PrfH Operon Protects E. coli ATCC25922 Strain from Colicin E3 Toxin | Maviza TP, Zarechenskaia AS, ..., Osterman IA | 10.3390/ijms23126453 |
38238085 | Characterization of tRNA splicing enzymes RNA ligase and tRNA2'-phosphotransferase from the pathogenic fungi Mucorales | Ghosh S, Dantuluri S, ..., Shuman S | 10.1261/rna.079911.123 |
37556111 | The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication bySuppressing Innate Immunity via Interaction with RNA Helicase DDX1 | Cao L, Hui X, ..., Jin M | 10.4049/jimmunol.2200799 |
35858322 | Sequential rescue and repair of stalled and damaged ribosome by bacterial PrfHand RtcB | Tian Y, Zeng F, ..., Huang RH | 10.1073/pnas.2202464119 |