Product Description: RuvB-like helicase 2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031011 | Ino80 complex |
| Component | GO:0035267 | NuA4 histone acetyltransferase complex |
| Component | GO:0097255 | R2TP complex |
| Component | GO:0000812 | Swr1 complex |
| Component | GO:0005737 | cytoplasm |
| Function | GO:0043139 | 5'-3' DNA helicase activity |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003678 | DNA helicase activity |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0006310 | DNA recombination |
| Process | GO:0006281 | DNA repair |
| Process | GO:0000492 | box C/D snoRNP assembly |
| Process | GO:0006338 | chromatin remodeling |
| Process | GO:0016573 | histone acetylation |
| Process | GO:0006357 | regulation of transcription by RNA polymerase II |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.40 | 0.80 |
| Trophozoite | expressed | 0.64 | 0.64 |
| Schizont | expressed | 0.08 | 0.95 |
| Gametocyte | expressed | 0.31 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1106000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.016 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0940900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIU3
"Best" AlphaFill ligand hit: 3AT (cordycepin triphosphate, Local RMSD=0.13) with 6K0R (Global RMSD=3.01)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9Y265 | RuvB-like 1 | HOGENOM, OrthoDB | CHEMBL3263107CHEMBL5178717CHEMBL3263107 |
Ortholog Group (OrthoMCL): OG6_101792
Most Similar Human Ortholog: J3QLR1
TM-align score: 0.95 | RMSD: 1.38
Seq Identity: 0.61 | Length: 130 / 475
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000175792 | RuvB like AAA ATPase 1 |
| ENSG00000284901 | RuvB like AAA ATPase 1 |
Protein Length: 475 | Molecular Weight (kDa): 53.406
PDB ID(s): None
Isoelectric Point: 7.09
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR010339 | TIP49, P-loop domain |
| InterPro | IPR027238 | RuvB-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR037938 | RuvB-like helicase 1 |
| InterPro | IPR041048 | RuvB-like, AAA-lid domain |
| PFam | PF06068 | TIP49, P-loop domain |
| PFam | PF17856 | RuvB-like, AAA-lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 30 | 18 | 39 |
| disruptive | 3 | 9 | 11 | 35 |
| missense | 3 | 9 | 10 | 31 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 24 | 75 | 13 | 26 |
| disruptive | 8 | 54 | 35 | 73 |
| missense | 5 | 37 | 24 | 46 |
PlasmoDB Total SNPs: 185
Non-coding: 151 | Synonymous: 26 | Nonsynonymous: 8 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 24161468 | Plasmodium falciparum RuvB2 translocates in 5'-3' direction, relocalizes duringschizont stage and its enzymatic activities are up regulated by RuvB3 of the samecomplex. | Ahmad M, Tuteja R | 10.1016/j.bbapap.2013.10.010 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 23219504 | Plasmodium falciparum RuvB1 is an active DNA helicase and translocates in the5'-3' direction | Ahmad M, Tuteja R | 10.1016/j.gene.2012.11.020 |