PF3D7_1106600

Genome location: Pf3D7_11_v3:275,163..279,995(+)

Genome classification: Core

Function and Localization

Product Description: DEAD/DEAH box helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	expressed	0.31	0.82
Schizont	not expressed	0.03	0.98
Gametocyte	not expressed	0.22	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1106600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.13 | MFS: -2.958 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIT7

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.01) with 6L5M (Global RMSD=3.53)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_134690

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	16	64	41	75
disruptive	41	171	104	249
missense	25	148	100	218

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	27	122	57	92
disruptive	89	370	176	333
missense	44	321	146	260

PlasmoDB Total SNPs: 198

Non-coding: 46 | Synonymous: 76 | Nonsynonymous: 76 | Stop Codon: 0

Protein Information

Protein Length: 1313 | Molecular Weight (kDa): 155.819

UniProt ID(s): Q8IIT7

PDB ID(s): None

Isoelectric Point: 8.53

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase