Product Description: DNA replication ATP-dependent helicase/nuclease DNA2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0004518 | nuclease activity |
| Process | GO:0006261 | DNA-dependent DNA replication |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.18 | 0.91 |
| Trophozoite | not expressed | 0.56 | 0.68 |
| Schizont | not expressed | 0.23 | 0.86 |
| Gametocyte | not expressed | 0.47 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1106700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.732 | #Insertions: 4
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0940200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0940200)
Modification: Disrupted | RMgm-2677
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIT6
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.05) with 2GK6 (Global RMSD=3.40)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102586
Most Similar Human Ortholog: A0A6Q8PHG1
TM-align score: 0.86 | RMSD: 1.68
Seq Identity: 0.26 | Length: 112 / 1024
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000132740 | immunoglobulin mu DNA binding protein 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 62 | 38 | 71 |
| disruptive | 15 | 105 | 66 | 160 |
| missense | 11 | 101 | 62 | 136 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 139 | 44 | 74 |
| disruptive | 42 | 226 | 107 | 230 |
| missense | 29 | 197 | 97 | 171 |
PlasmoDB Total SNPs: 86
Non-coding: 22 | Synonymous: 34 | Nonsynonymous: 28 | Stop Codon: 2
Protein Length: 1024 | Molecular Weight (kDa): 120.147
UniProt ID(s): Q8IIT6
PDB ID(s): None
Isoelectric Point: 8.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR041677 | DNA2/NAM7 helicase, helicase domain |
| InterPro | IPR041679 | DNA2/NAM7 helicase-like, C-terminal |
| PFam | PF13086 | DNA2/NAM7 helicase, helicase domain |
| PFam | PF13087 | DNA2/NAM7 helicase-like, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 37956214 | Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA fromdegradation by Sgs1-Dna2 | Gali VK, Monerawela C, ..., Donaldson AD | 10.1371/journal.pgen.1011044 |