Product Description: pseudo-tyrosine kinase-like protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.10.2 (Non-specific protein-tyrosine kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0044164 | host cell cytosol |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0005524 | ATP binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0004672 | protein kinase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.16 | 0.92 |
| Trophozoite | expressed | 0.66 | 0.64 |
| Schizont | expressed | 0.24 | 0.86 |
| Gametocyte | expressed | 0.75 | 0.60 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1106800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.998 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0940100)
Modification: Disrupted | RMgm-2676
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIT5
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.06) with 6P7G (Global RMSD=2.45)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.10.2 | non-specific protein-tyrosine kinase | ADPPP1PP2T47ChkCskPIP2CMP6RNAiWASPmorinAG490... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 84 | 48 | 98 |
| disruptive | 89 | 249 | 135 | 288 |
| missense | 43 | 189 | 106 | 243 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 37 | 157 | 72 | 87 |
| disruptive | 167 | 484 | 204 | 362 |
| missense | 85 | 364 | 169 | 265 |
PlasmoDB Total SNPs: 473
Non-coding: 159 | Synonymous: 176 | Nonsynonymous: 138 | Stop Codon: 0
Protein Length: 1501 | Molecular Weight (kDa): 176.12
UniProt ID(s): Q8IIT5
PDB ID(s): None
Isoelectric Point: 9.97
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001245 | Serine-threonine/tyrosine-protein kinase, catalytic domain |
| InterPro | IPR001660 | Sterile alpha motif domain |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR013761 | Sterile alpha motif/pointed domain superfamily |
| PFam | PF07647 | Sterile alpha motif domain |
| PFam | PF07714 | Serine-threonine/tyrosine-protein kinase, catalytic domain |
| Superfamily | SSF47769 | Sterile alpha motif/pointed domain superfamily |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| Superfamily | SSF82185 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 31148576 | Plasmodium pseudo-Tyrosine Kinase-like binds PP1 and SERA5 and is exported tohost erythrocytes. | Gnangnon B, Freville A, ..., Pierrot C | 10.1038/s41598-019-44542-3 |