PF3D7_1107100

Genome location: Pf3D7_11_v3:293,534..297,356(+)

Genome classification: Core

Function and Localization

Product Description: nucleic acid binding protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016607	nuclear speck
Component	GO:0005634	nucleus
Function	GO:0003723	RNA binding
Function	GO:0003729	mRNA binding
Function	GO:0003676	nucleic acid binding
Process	GO:0045292	mRNA cis splicing, via spliceosome
Process	GO:0006139	nucleobase-containing compound metabolic process
Process	GO:0000381	regulation of alternative mRNA splicing, via spliceosome

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.04	0.98
Trophozoite	expressed	0.32	0.80
Schizont	expressed	0.78	0.58
Gametocyte	expressed	0.92	0.52

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1107100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.612 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1103000)

Relative Growth Rate: 0.14 ± 0.22
Confidence: 4.40

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0939800)

Modification: Disrupted | RMgm-2674

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIT2

"Best" AlphaFill ligand hit: VIB (3-(4-amino-2-methyl-pyrimidin-5-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium, Local RMSD=10.40) with 2HH9 (Global RMSD=32.59)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104633

Most Similar Human Ortholog: J3QL05

TM-align score: 0.64 | RMSD: 3.35

Seq Identity: 0.18 | Length: 104 / 588

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000161547	serine and arginine rich splicing factor 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	36	24	42
disruptive	14	100	41	122
missense	12	96	39	109

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	68	23	42
disruptive	32	189	88	185
missense	23	169	76	126

PlasmoDB Total SNPs: 313

Non-coding: 274 | Synonymous: 19 | Nonsynonymous: 20 | Stop Codon: 0

Protein Information

Protein Length: 588 | Molecular Weight (kDa): 69.259

UniProt ID(s): Q8IIT2

PDB ID(s): None

Isoelectric Point: 9.68

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000504	RNA recognition motif domain
InterPro	IPR035979	RNA-binding domain superfamily
InterPro	IPR037366	BOULE/DAZ family
PFam	PF00076	RNA recognition motif domain
Superfamily	SSF54928	RNA-binding domain superfamily