Product Description: DNA repair protein RAD51
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000794 | condensed nuclear chromosome |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0008094 | ATP-dependent activity, acting on DNA |
| Function | GO:0003677 | DNA binding |
| Function | GO:0000150 | DNA strand exchange activity |
| Function | GO:0003684 | damaged DNA binding |
| Function | GO:0003690 | double-stranded DNA binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Process | GO:0000730 | DNA recombinase assembly |
| Process | GO:0006310 | DNA recombination |
| Process | GO:0006281 | DNA repair |
| Process | GO:0070192 | chromosome organization involved in meiotic cell cycle |
| Process | GO:0097551 | mitochondrial double-strand break repair |
| Process | GO:0006312 | mitotic recombination |
| Process | GO:0007131 | reciprocal meiotic recombination |
| Process | GO:0010212 | response to ionizing radiation |
| Process | GO:0042148 | strand invasion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.01 | 0.99 |
| Trophozoite | expressed | 0.08 | 0.94 |
| Schizont | expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.20 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1107400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.307 | MFS: -2.691 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0714000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIS8
"Best" AlphaFill ligand hit: DTP (, Local RMSD=0.20) with 2G88 (Global RMSD=4.17)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q06609 | DNA repair protein RAD51 homolog 1 | Homo sapiens | OMA, OrthoDB | MLS000727607cid_6602691Salazinic acid, 2Stictic acid (StA), 1... |
Ortholog Group (OrthoMCL): OG6_100483
Most Similar Human Ortholog: Q06609
TM-align score: 0.96 | RMSD: 1.99
Seq Identity: 0.61 | Length: 339 / 350
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000051180 | RAD51 recombinase |
| ENSG00000100206 | DNA meiotic recombinase 1 |
Protein Length: 350 | Molecular Weight (kDa): 38.592
PDB ID(s): None
Isoelectric Point: 7.03
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR010995 | DNA repair Rad51/transcription factor NusA, alpha-helical |
| InterPro | IPR011941 | DNA recombination/repair protein Rad51 |
| InterPro | IPR013632 | DNA recombination and repair protein Rad51-like, C-terminal |
| InterPro | IPR016467 | DNA recombination and repair protein, RecA-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR033925 | Rad51/DMC1/RadA |
| PFam | PF08423 | DNA recombination and repair protein Rad51-like, C-terminal |
| Superfamily | SSF47794 | DNA repair Rad51/transcription factor NusA, alpha-helical |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 26 | 12 | 16 |
| disruptive | 2 | 3 | 1 | 12 |
| missense | 2 | 2 | 1 | 6 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 47 | 9 | 23 |
| disruptive | 3 | 15 | 20 | 71 |
| missense | 3 | 9 | 16 | 48 |
PlasmoDB Total SNPs: 125
Non-coding: 115 | Synonymous: 9 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15610817 | Characterization of kinetics of DNA strand-exchange and ATP hydrolysis activitiesof recombinant PfRad51, a Plasmodium falciparum recombinase. | Bhattacharyya MK, Bhattacharyya nee Deb S, Jayabalasingham B, Kumar N | 10.1016/j.molbiopara.2004.09.007 |
| 23631919 | Opposing roles for two molecular forms of replication protein A inRad51-Rad54-mediated DNA recombination in Plasmodium falciparum. | Gopalakrishnan AM, Kumar N | 10.1128/mBio.02515-14 |
| 33239368 | Elucidation of D NA Repair Function of Pf Blm and Potentiation of Artemisinin Action by a Small-Molecule Inhibitor of Rec Q Helicase | Suthram N, Padhi S, ..., Bhattacharyya MK | 10.1128/mSphere.00956-20 |
| 34730376 | Bloom Helicase Along with Recombinase Rad51 Repairs the Mitochondrial Genome ofthe Malaria Parasite. | Jha P, Gahlawat A, ..., Bhattacharyya MK | 10.1128/mSphere.00718-21 |