PF3D7_1107900 (MSCS)

Genome location: Pf3D7_11_v3:343,913..349,351(-)

Genome classification: Core

Function and Localization

Product Description: mechanosensitive ion channel protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005886	plasma membrane
Function	GO:0008381	mechanosensitive ion channel activity
Process	GO:0006820	anion transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.11	0.94
Trophozoite	not expressed	0.18	0.90
Schizont	possibly expressed	0.31	0.81
Gametocyte	expressed	0.72	0.62

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1107900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 5 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.395 | #Insertions: 4

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0939000)

Modification: Disrupted | RMgm-1381

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIS3

"Best" AlphaFill ligand hit: HEX (hexane, Local RMSD=0.02) with 5AJI (Global RMSD=5.16)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_124595

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	112	69	147
disruptive	39	206	104	258
missense	26	185	92	227

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	36	223	95	141
disruptive	99	459	191	442
missense	59	376	155	318

PlasmoDB Total SNPs: 178

Non-coding: 0 | Synonymous: 129 | Nonsynonymous: 49 | Stop Codon: 0

Protein Information

Protein Length: 1812 | Molecular Weight (kDa): 213.622

UniProt ID(s): Q8IIS3

PDB ID(s): None

Isoelectric Point: 9.14

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006685	Mechanosensitive ion channel MscS
InterPro	IPR010920	LSM domain superfamily
PFam	PF00924	Mechanosensitive ion channel MscS
Superfamily	SSF50182	LSM domain superfamily