PF3D7_1108300 (PCD)

Genome location: Pf3D7_11_v3:362,243..363,831(+)

Genome classification: Core

Function and Localization

Product Description: pterin-4a-carbinolamine dehydratase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 4.2.1.96 (4a-hydroxytetrahydrobiopterin dehydratase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0008124	4-alpha-hydroxytetrahydrobiopterin dehydratase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.05	0.97
Trophozoite	no data	0.19	0.87
Schizont	no data	0.09	0.94
Gametocyte	no data	0.26	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1108300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.916 | MFS: -0.971 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6S3E6

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.2.1.96	4a-hydroxytetrahydrobiopterin dehydratase	glyceroldiethyldicarbonateQuinoid 6(S)-propyldihydropterinQuinoid 6(S)-methyldihydropterin

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100957

Most Similar Human Ortholog: P61457

TM-align score: 0.83 | RMSD: 1.50

Seq Identity: 0.27 | Length: 95 / 107

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000132570	pterin-4 alpha-carbinolamine dehydratase 2
ENSG00000166228	pterin-4 alpha-carbinolamine dehydratase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 125

Non-coding: 121 | Synonymous: 1 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 107 | Molecular Weight (kDa): 12.937

UniProt ID(s): C6S3E6, Q2Q450

PDB ID(s): None

Isoelectric Point: 9.55

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001533	Pterin 4 alpha carbinolamine dehydratase
InterPro	IPR036428	Pterin 4 alpha carbinolamine dehydratase superfamily
PFam	PF01329	Pterin 4 alpha carbinolamine dehydratase
Superfamily	SSF55248	Pterin 4 alpha carbinolamine dehydratase superfamily