Genome location: Pf3D7_11_v3:363,945..366,461(+)
Genome classification: Core
Product Description: casein kinase 2, alpha subunit
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0005634 | nucleus |
| Component | GO:0005956 | protein kinase CK2 complex |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0018105 | peptidyl-serine phosphorylation |
| Process | GO:0018107 | peptidyl-threonine phosphorylation |
| Process | GO:0046777 | protein autophosphorylation |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0051726 | regulation of cell cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.71 | 0.66 |
| Trophozoite | expressed | 1.19 | 0.41 |
| Schizont | expressed | 0.34 | 0.79 |
| Gametocyte | expressed | 0.58 | 0.69 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1108400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.134 | MFS: -3.148 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0938600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_0941100)
Modification: Tagged | RMgm-1559
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIR9
"Best" AlphaFill ligand hit: V55 (4-hydroxy-3-methoxybenzaldehyde, Local RMSD=0.12) with 6HOP (Global RMSD=0.82)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P19139 | Casein kinase II subunit alpha/beta | HOGENOM, OMA, OrthoDB | 4,5,6,7-tetraiodobenzimidazole (TIBI), 1aiodinated benzimidazole, 2aiodinated benzimidazole, 3aiodinated benzimidazole, 4a... | |
| P40231 | Casein kinase II subunit alpha | Schizosaccharomyces pombe | HOGENOM, OMA, OrthoMCL | CHEMBL410456 |
| P68400 | Casein kinase II subunit alpha | Homo sapiens | HOGENOM, OMA, OrthoDB | CGP029482CGP06028CGP060476CGP062644... |
| P28523 | Casein kinase II subunit alpha | Zea mays | HOGENOM, OMA, OrthoDB, OrthoMCL | MNAMNXDAAmed.21724, Compound 24... |
| Q8NEV1 | Casein kinase II subunit alpha 3 | HOGENOM, OrthoDB | CHEMBL4284969CHEMBL1230165cid_5281607Fisetin (14)... | |
| P19784 | Casein kinase II subunit alpha' | Homo sapiens | OrthoDB | US8791257, 4US8791257, 17US8791257, 26US8791257, 28... |
Ortholog Group (OrthoMCL): OG6_100821
Most Similar Human Ortholog: P19784
TM-align score: 0.98 | RMSD: 0.92
Seq Identity: 0.62 | Length: 332 / 335
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000070770 | casein kinase 2 alpha 2 |
| ENSG00000101266 | casein kinase 2 alpha 1 |
| ENSG00000254598 | casein kinase 2 alpha 3 |
Protein Length: 335 | Molecular Weight (kDa): 39.89
UniProt ID(s): Q8IIR9
PDB ID(s): 5XVU
Isoelectric Point: 9.08
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 22 | 28 | 24 |
| disruptive | 2 | 3 | 0 | 10 |
| missense | 2 | 2 | 0 | 9 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 61 | 14 | 18 |
| disruptive | 4 | 12 | 14 | 54 |
| missense | 2 | 7 | 11 | 39 |
PlasmoDB Total SNPs: 70
Non-coding: 65 | Synonymous: 4 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19114502 | Functional analysis of protein kinase CK2 of the human malaria parasitePlasmodium falciparum. | Holland Z, Prudent R, ..., Doerig C | 10.1128/EC.00334-08 |
| 22293287 | Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assemblypathway. | Dastidar EG, Dayer G, ..., Doerig C | 10.1186/1741-7007-10-5 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24658579 | Malaria protein kinase CK2 (PfCK2) shows novel mechanisms of regulation. | Graciotti M, Alam M, ..., Tobin AB | 10.1371/journal.pone.0085391 |
| 26629826 | Malaria Parasite-Infected Erythrocytes Secrete PfCK1, the Plasmodium Homologue ofthe Pleiotropic Protein Kinase Casein Kinase 1. | Dorin-Semblat D, Demarta-Gatsi C, ..., Doerig C | 10.1371/journal.pone.0139591 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31723180 | Mapping and functional analysis of heterochromatin protein 1 phosphorylation inthe malaria parasite Plasmodium falciparum. | Bui HTN, Niederwieser I, ..., Voss TS | 10.1038/s41598-019-53325-9 |
| 33712726 | The catalytic subunit of Plasmodium falciparum casein kinase 2 is essential forgametocytogenesis. | Hitz E, Gruninger O, ..., Voss TS | 10.1038/s42003-021-01873-0 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 37944834 | Ginsenoside Rh1, a novel casein kinase II subunit alpha (CK2alpha) inhibitor, retardsmetastasis via disrupting HHEX/CCL20 signaling cascade involved in tumor cellextravasation across endothelial barrier | Zheng W, Shen P, ..., Zhao Y | 10.1016/j.phrs.2023.106986 |
| 37907238 | Cell cycle-dependent gene networks for cell proliferation activated by nuclear CK2alpha complexes | Homma MK, Nakato R, ..., Homma Y | 10.26508/lsa.202302077 |
| 37594910 | Inhibition of CK2alpha accelerates skin wound healing by promoting endothelial cell proliferation through the Hedgehog signaling pathway | Zhu J, Chen P, ..., Guan X | 10.1096/fj.202300478RR |