Genome location: Pf3D7_11_v3:366,977..369,608(+)
Genome classification: Core
Product Description: succinate--CoA ligase [ADP-forming] subunit alpha, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.2.1.4 (Succinate--CoA ligase (GDP-forming));6.2.1.5 (Succinate--CoA ligase (ADP-forming))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005759 | mitochondrial matrix |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0009361 | succinate-CoA ligase complex (ADP-forming) |
| Component | GO:0045244 | succinate-CoA ligase complex (GDP-forming) |
| Function | GO:0004775 | succinate-CoA ligase (ADP-forming) activity |
| Function | GO:0004776 | succinate-CoA ligase (GDP-forming) activity |
| Process | GO:0006104 | succinyl-CoA metabolic process |
| Process | GO:0006099 | tricarboxylic acid cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.32 | 0.80 |
| Schizont | expressed | 0.28 | 0.82 |
| Gametocyte | expressed | 0.34 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1108500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 1.366 | #Insertions: 3
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0938500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0938500)
Modification: Disrupted | RMgm-2669
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIR8
"Best" AlphaFill ligand hit: COA (coenzyme a, Local RMSD=0.07) with 2NU7 (Global RMSD=1.84)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100834
Most Similar Human Ortholog: P53597
TM-align score: 0.90 | RMSD: 1.49
Seq Identity: 0.63 | Length: 300 / 327
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000163541 | succinate-CoA ligase GDP/ADP-forming subunit alpha |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 26 | 9 | 30 |
| disruptive | 3 | 18 | 13 | 33 |
| missense | 3 | 18 | 13 | 28 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 49 | 13 | 33 |
| disruptive | 5 | 39 | 16 | 70 |
| missense | 4 | 36 | 14 | 48 |
PlasmoDB Total SNPs: 137
Non-coding: 122 | Synonymous: 12 | Nonsynonymous: 2 | Stop Codon: 1
Protein Length: 327 | Molecular Weight (kDa): 35.396
UniProt ID(s): Q8IIR8
PDB ID(s): None
Isoelectric Point: 8.72
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003781 | CoA-binding |
| InterPro | IPR005810 | Succinyl-CoA ligase, alpha subunit |
| InterPro | IPR005811 | ATP-citrate lyase/succinyl-CoA ligase |
| InterPro | IPR016102 | Succinyl-CoA synthetase-like |
| InterPro | IPR017440 | ATP-citrate lyase/succinyl-CoA ligase, active site |
| InterPro | IPR033847 | ATP-citrate lyase/succinyl-CoA ligase, conserved site |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF00549 | ATP-citrate lyase/succinyl-CoA ligase |
| PFam | PF02629 | CoA-binding |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
| Superfamily | SSF52210 | Succinyl-CoA synthetase-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |