PF3D7_1110200 (PRPF6)

Genome location: Pf3D7_11_v3:405,087..410,073(+)

Genome classification: Core

Function and Localization

Product Description: pre-mRNA-processing factor 6, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0046540	U4/U6 x U5 tri-snRNP complex
Component	GO:0071013	catalytic step 2 spliceosome
Process	GO:0000398	mRNA splicing, via spliceosome
Process	GO:0000244	spliceosomal tri-snRNP complex assembly

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.06	0.52
Trophozoite	expressed	0.76	0.58
Schizont	expressed	0.45	0.72
Gametocyte	expressed	0.47	0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1110200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.871 | MFS: -2.907 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0937400)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIR0

"Best" AlphaFill ligand hit: FMN (flavin mononucleotide, Local RMSD=0.70) with 1TV5 (Global RMSD=13.80)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102682

Most Similar Human Ortholog: O94906

TM-align score: 0.55 | RMSD: 6.23

Seq Identity: 0.35 | Length: 641 / 1329

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000101161	pre-mRNA processing factor 6

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	24	78	47	98
disruptive	23	122	76	185
missense	18	116	74	174

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	32	167	69	93
disruptive	42	261	128	272
missense	34	232	116	226

PlasmoDB Total SNPs: 233

Non-coding: 132 | Synonymous: 69 | Nonsynonymous: 31 | Stop Codon: 1

Protein Information

Protein Length: 1329 | Molecular Weight (kDa): 152.15

UniProt ID(s): Q8IIR0

PDB ID(s): None

Isoelectric Point: 9.15

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003107	HAT (Half-A-TPR) repeat
InterPro	IPR010491	PRP1 splicing factor, N-terminal
InterPro	IPR011990	Tetratricopeptide-like helical domain superfamily
InterPro	IPR027108	Pre-mRNA-processing factor 6/Prp1/STA1
PFam	PF06424	PRP1 splicing factor, N-terminal
Superfamily	SSF48452	Tetratricopeptide-like helical domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104