About Gene List

PF3D7_1110500 (VPS35)

Genome location: Pf3D7_11_v3:421,374..425,853(+)

Genome classification: Core

Function and Localization

Product Description: vacuolar protein sorting-associated protein 35, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005770 late endosome
Component GO:0030117 membrane coat
Component GO:0005634 nucleus
Component GO:0030904 retromer complex
Process GO:0006886 intracellular protein transport
Process GO:0042147 retrograde transport, endosome to Golgi

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.13 0.93
Trophozoite expressed 0.60 0.65
Schizont expressed 0.32 0.79
Gametocyte expressed 0.55 0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1110500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.126 | MFS: -2.866 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0937100)

  • Relative Growth Rate: 0.10 ± nan
  • Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIQ6

"Best" AlphaFill ligand hit: HEM (heme, Local RMSD=0.17) with 7KVI (Global RMSD=15.45)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101945

Most Similar Human Ortholog: Q96QK1

TM-align score: 0.74 | RMSD: 5.57

Seq Identity: 0.20 | Length: 743 / 1050

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000069329 VPS35 retromer complex component

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 9 63 23 70
disruptive 20 92 67 154
missense 13 76 52 121

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 26 125 46 81
disruptive 39 249 130 325
missense 23 184 101 209

PlasmoDB Total SNPs: 179

Non-coding: 70 | Synonymous: 84 | Nonsynonymous: 25 | Stop Codon: 0

Protein Information

Protein Length: 1050 | Molecular Weight (kDa): 123.468

UniProt ID(s): Q8IIQ6

PDB ID(s): None

Isoelectric Point: 6.34

Protein Domain Annotations:

Source Family ID Description
InterPro IPR005378 Vacuolar protein sorting-associated protein 35
PFam PF03635 Vacuolar protein sorting-associated protein 35
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
29654975 Detection of retromer assembly in Plasmodium falciparum by immunosensing coupledto Surface Plasmon Resonance. Iqbal MS, Siddiqui AA, ..., Bandyopadhyay U https://pubmed.ncbi.nlm.nih.gov/29654975/