Product Description: RNA cytosine C(5)-methyltransferase NSUN2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.29 (Transferred entry: 2.1.1.202, 2.1.1.203 and 2.1.1.204)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005762 | mitochondrial large ribosomal subunit |
| Component | GO:0005634 | nucleus |
| Function | GO:0008168 | methyltransferase activity |
| Function | GO:0016428 | tRNA (cytosine-5-)-methyltransferase activity |
| Process | GO:0001510 | RNA methylation |
| Process | GO:0031167 | rRNA methylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.08 | 0.95 |
| Schizont | possibly expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1111000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -2.971 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0936600)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PY17X_0938600)
Modification: Tagged | RMgm-5040
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIQ2
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.16) with 5ZVE (Global RMSD=6.17)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.29 | tRNA (cytosine-5-)-methyltransferase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_104090
Most Similar Human Ortholog: Q9H649
TM-align score: 0.77 | RMSD: 3.18
Seq Identity: 0.27 | Length: 298 / 579
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000178694 | NOP2/Sun RNA methyltransferase 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 31 | 16 | 29 |
| disruptive | 37 | 114 | 56 | 121 |
| missense | 23 | 93 | 47 | 107 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 61 | 21 | 26 |
| disruptive | 62 | 210 | 88 | 191 |
| missense | 37 | 174 | 75 | 106 |
PlasmoDB Total SNPs: 101
Non-coding: 33 | Synonymous: 40 | Nonsynonymous: 28 | Stop Codon: 0
Protein Length: 579 | Molecular Weight (kDa): 68.586
UniProt ID(s): Q8IIQ2
PDB ID(s): None
Isoelectric Point: 7.91
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001678 | SAM-dependent methyltransferase RsmB/NOP2-type |
| InterPro | IPR023267 | RNA (C5-cytosine) methyltransferase |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF01189 | SAM-dependent methyltransferase RsmB/NOP2-type |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 35210361 | 5-methylcytosine modification by Plasmodium N SUN2 stabilizes m RNA and mediatesthe development of gametocytes | Liu M, Guo G, ..., Zhang Q | 10.1073/pnas.2110713119 |