PF3D7_1111900

Genome location: Pf3D7_11_v3:457,356..459,438(+)

Genome classification: Core

Function and Localization

Product Description: Josephin domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.19.12 (Ubiquitinyl hydrolase 1)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004843	thiol-dependent deubiquitinase
Process	GO:0016579	protein deubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.99
Trophozoite	not expressed	0.24	0.84
Schizont	not expressed	0.15	0.89
Gametocyte	not expressed	0.39	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1111900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.295 | MFS: -1.886 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIP4

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=2.99) with 4XE8 (Global RMSD=18.27)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.19.12	ubiquitinyl hydrolase 1	KClNEMDTTEDTAEGTARNAisiRNApeptidesAntibodyubiquitinLDN-574442-propenal...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104335

Most Similar Human Ortholog: M0QZL8

TM-align score: 0.78 | RMSD: 2.53

Seq Identity: 0.23 | Length: 146 / 236

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000100221	Josephin domain containing 1
ENSG00000161677	Josephin domain containing 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	9	5	17
disruptive	3	21	10	25
missense	3	21	10	23

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	16	17	11
disruptive	3	56	20	46
missense	3	50	18	29

PlasmoDB Total SNPs: 111

Non-coding: 98 | Synonymous: 9 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 236 | Molecular Weight (kDa): 28.285

UniProt ID(s): Q8IIP4

PDB ID(s): None

Isoelectric Point: 8.15

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006155	Josephin domain
InterPro	IPR040053	Josephin-1/2
PFam	PF02099	Josephin domain
Superfamily	N/A	N/A