PF3D7_1112100

Genome location: Pf3D7_11_v3:461,766..469,974(+)

Genome classification: Core

Function and Localization

Product Description: protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 3

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000778	condensed nuclear chromosome kinetochore
Function	GO:0004672	protein kinase activity
Process	GO:0051754	meiotic sister chromatid cohesion, centromeric
Process	GO:0007094	mitotic spindle assembly checkpoint signaling

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.48	0.78
Trophozoite	expressed	1.09	0.47
Schizont	expressed	1.97	0.20
Gametocyte	expressed	0.87	0.55

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1112100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.946 | MFS: -2.931 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0935500)

Relative Growth Rate: 0.08 ± 0.26
Confidence: 4.08

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIP2

"Best" AlphaFill ligand hit: IPH (phenol, Local RMSD=0.02) with 6Q7S (Global RMSD=17.93)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533593

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	28	110	65	137
disruptive	154	333	179	423
missense	81	237	154	361

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	42	216	92	147
disruptive	242	596	255	572
missense	125	461	211	425

PlasmoDB Total SNPs: 356

Non-coding: 42 | Synonymous: 165 | Nonsynonymous: 147 | Stop Codon: 2

Protein Information

Protein Length: 2460 | Molecular Weight (kDa): 296.625

UniProt ID(s): Q8IIP2

PDB ID(s): None

Isoelectric Point: 9.57

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR015661	Mitotic spindle checkpoint protein Bub1/Mad3
PFam	N/A	N/A
Superfamily	SSF56112	Protein kinase-like domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
12368868	Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14.	Gardner MJ, Shallom SJ, ..., Fraser CM	10.1038/nature01094
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104