Product Description: ATPase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0008047 | enzyme activator activity |
| Function | GO:0017116 | single-stranded DNA helicase activity |
| Process | GO:0006261 | DNA-dependent DNA replication |
| Process | GO:0006282 | regulation of DNA repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.97 |
| Trophozoite | not expressed | 0.41 | 0.75 |
| Schizont | possibly expressed | 0.27 | 0.83 |
| Gametocyte | not expressed | 0.74 | 0.61 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1112600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.134 | MFS: -2.843 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIN7
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.34) with 1IN4 (Global RMSD=16.70)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102902
Most Similar Human Ortholog: Q96S55
TM-align score: 0.48 | RMSD: 5.57
Seq Identity: 0.20 | Length: 410 / 707
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000124535 | WRN helicase interacting protein 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 31 | 22 | 46 |
| disruptive | 29 | 71 | 45 | 98 |
| missense | 18 | 52 | 36 | 80 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 58 | 34 | 47 |
| disruptive | 52 | 168 | 66 | 174 |
| missense | 31 | 120 | 48 | 121 |
PlasmoDB Total SNPs: 148
Non-coding: 75 | Synonymous: 49 | Nonsynonymous: 24 | Stop Codon: 0
Protein Length: 707 | Molecular Weight (kDa): 82.414
UniProt ID(s): Q8IIN7
PDB ID(s): None
Isoelectric Point: 7.26
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002078 | RNA polymerase sigma factor 54 interaction domain |
| InterPro | IPR008921 | DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal |
| InterPro | IPR021886 | MgsA AAA+ ATPase C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF12002 | MgsA AAA+ ATPase C-terminal |
| PFam | PF14532 | RNA polymerase sigma factor 54 interaction domain |
| Superfamily | SSF48019 | DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |