PF3D7_1113400 (DSK2)

Genome location: Pf3D7_11_v3:521,020..522,963(-)

Genome classification: Core

Function and Localization

Product Description: ubiquitin domain-containing protein DSK2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Function	GO:0031593	polyubiquitin modification-dependent protein binding
Process	GO:0030433	ubiquitin-dependent ERAD pathway
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.45	0.77
Trophozoite	expressed	0.44	0.73
Schizont	expressed	0.27	0.81
Gametocyte	expressed	0.31	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1113400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.752 | MFS: -2.802 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0916400)

Relative Growth Rate: 0.82 ± 0.10
Confidence: 5.92

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIM8

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100933

Most Similar Human Ortholog: Q9UMX0

TM-align score: 0.30 | RMSD: 5.93

Seq Identity: 0.16 | Length: 166 / 388

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000135018	ubiquilin 1
ENSG00000160803	ubiquilin 4
ENSG00000188021	ubiquilin 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	14	12	34
disruptive	3	18	14	51
missense	2	16	14	43

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	42	24	32
disruptive	6	50	40	102
missense	5	43	36	87

PlasmoDB Total SNPs: 116

Non-coding: 103 | Synonymous: 9 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 388 | Molecular Weight (kDa): 42.706

UniProt ID(s): Q8IIM8

PDB ID(s): 6JL3

Isoelectric Point: 4.33

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000626	Ubiquitin-like domain
InterPro	IPR009060	UBA-like superfamily
InterPro	IPR015496	Ubiquilin
InterPro	IPR015940	Ubiquitin-associated domain
InterPro	IPR029071	Ubiquitin-like domain superfamily
PFam	PF00240	Ubiquitin-like domain
PFam	PF00627	Ubiquitin-associated domain
Superfamily	SSF46934	UBA-like superfamily
Superfamily	SSF54236	Ubiquitin-like domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
32057831	The recognition of proteasomal receptors by Plasmodium falciparum DSK2	Gupta I, Khan S	10.1016/j.molbiopara.2020.111266
32808330	Toxoplasma gondii UBL-UBA shuttle proteins contribute to the degradation ofubiquitinylated proteins and are important for synchronous cell division andvirulence	Zhang H, Liu J, ..., Liu Q	10.1096/fj.202000759RR
34727385	Toxoplasma gondii UBL-UBA shuttle proteins regulate several important cellularprocesses	Zhang H, Xue Y, ..., Liu Q	10.1096/fj.202100662RR