Product Description: glyoxalase I
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.4.1.5 (Lactoylglutathione lyase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Function | GO:0004462 | lactoylglutathione lyase activity |
| Process | GO:0006749 | glutathione metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.28 | 0.86 |
| Trophozoite | not expressed | 0.60 | 0.66 |
| Schizont | not expressed | 0.06 | 0.96 |
| Gametocyte | not expressed | 0.21 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1113700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.234 | MFS: -2.7 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0933900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0933900)
Modification: Disrupted | RMgm-2649
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIM5
"Best" AlphaFill ligand hit: ZST (3,4-dihydro-4-oxo-3-((5-trifluoromethyl-2-benzothiazolyl)methyl)-1-phthalazine acetic acid, Local RMSD=0.11) with 4KYH (Global RMSD=3.04)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.4.1.5 | lactoylglutathione lyase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P50107 | Glyoxalase I | Saccharomyces cerevisiae | HOGENOM, OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | CHEMBL128872CHEMBL221011CHEMBL218644CHEMBL128872... |
| Q04760 | Lactoylglutathione lyase | OrthoDB, OrthoMCL BLAST | CHEMBL218644CHEMBL159603CHEMBL218644S-(N-Methyl-N-hydroxycarbomyl)ethylglutathione... | |
| Q9CPU0 | Lactoylglutathione lyase | OrthoDB, OrthoMCL BLAST | CHEMBL1234300glycyrrhetinic acidCHEMBL4584432 |
Ortholog Group (OrthoMCL): OG6_102622
Most Similar Human Ortholog: Q04760
TM-align score: 0.78 | RMSD: 2.97
Seq Identity: 0.44 | Length: 164 / 356
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000124767 | glyoxalase I |
Protein Length: 356 | Molecular Weight (kDa): 42.441
PDB ID(s): None
Isoelectric Point: 6.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004360 | Glyoxalase/fosfomycin resistance/dioxygenase domain |
| InterPro | IPR018146 | Glyoxalase I, conserved site |
| InterPro | IPR029068 | Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase |
| PFam | PF00903 | Glyoxalase/fosfomycin resistance/dioxygenase domain |
| Superfamily | SSF54593 | Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 20 | 9 | 22 |
| disruptive | 2 | 25 | 14 | 47 |
| missense | 2 | 25 | 14 | 43 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 32 | 18 | 22 |
| disruptive | 7 | 63 | 27 | 76 |
| missense | 7 | 51 | 23 | 51 |
PlasmoDB Total SNPs: 69
Non-coding: 53 | Synonymous: 8 | Nonsynonymous: 8 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 14623080 | Glyoxalase I of the malarial parasite Plasmodium falciparum: evidence for subunitfusion. | Iozef R, Rahlfs S, ..., Becker K | 10.1016/s0014-5793(03)01146-3 |
| 15843146 | Characterization of the glyoxalases of the malarial parasite Plasmodiumfalciparum and comparison with their human counterparts. | Akoachere M, Iozef R, ..., Becker K | 10.1515/BC.2005.006 |
| 22607473 | Tight-binding inhibitors efficiently inactivate both reaction centers ofmonomeric Plasmodium falciparum glyoxalase 1. | Urscher M, More SS, ..., Deponte M | 10.1111/j.1742-4658.2012.08640.x |
| 29354648 | The cytosolic glyoxalases of Plasmodium falciparum are dispensable during asexualblood-stage development. | Wezena CA, Alisch R, ..., Deponte M | 10.15698/mic2018.01.608 |