PF3D7_1114100 (ROM1)

Genome location: Pf3D7_11_v3:539,792..542,025(+)

Genome classification: Core

Function and Localization

Product Description: rhomboid protease ROM1

SignalP Peptide: N/A

# Transmembrane Domains: 7

EC Numbers: 3.4.21.105 (Rhomboid protease)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020009	microneme
Function	GO:0008233	peptidase activity
Process	GO:0006465	signal peptide processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	nd	nd
Trophozoite	expressed	nd	nd
Schizont	expressed	nd	nd
Gametocyte	expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1114100.2

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.274 | MFS: -2.546 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0933500)

Relative Growth Rate: 1.01 ± 0.08
Confidence: 6.39

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0933500)

Modification: Disrupted | RMgm-2645

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIM2

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.21.105	rhomboid protease	dodecyl maltosidedichloroisocoumarin3,4-dichloroisocoumarinn-nonyl-beta-D-glucosidediisopropyl fluorophosphonate3,4-dichloro-1H-2-benzopyran-1-one7-amino-4-chloro-3-methoxyisocoumarin3-butyl-4-(pent-4-yn-1-yl)oxetan-2-one1-myristoyl-sn-glycero-3-phosphocholine1,2-dihexanoyl-sn-glycero-3-phosphocholine1,2-dimyristoyl-sn-glycero-3-phosphocholinephenyl 2-oxo-4-phenylazetidine-1-carboxylate...
3.4.21.105	rhomboid protease	dodecyl maltosidedichloroisocoumarin3,4-dichloroisocoumarinn-nonyl-beta-D-glucosidediisopropyl fluorophosphonate3,4-dichloro-1H-2-benzopyran-1-one7-amino-4-chloro-3-methoxyisocoumarin3-butyl-4-(pent-4-yn-1-yl)oxetan-2-one1-myristoyl-sn-glycero-3-phosphocholine1,2-dihexanoyl-sn-glycero-3-phosphocholine1,2-dimyristoyl-sn-glycero-3-phosphocholinephenyl 2-oxo-4-phenylazetidine-1-carboxylate...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9WZ53	Rhomboid domain-containing protein		OrthoMCL, OrthoMCL BLAST	cid_1609
P54493	Rhomboid protease GluP		OrthoMCL, OrthoMCL BLAST	cid_1609CHEMBL4225584CHEMBL4225103CHEMBL79597...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100562

Most Similar Human Ortholog: Q96CC6

TM-align score: 0.72 | RMSD: 3.49

Seq Identity: 0.25 | Length: 241 / 278

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000007384	rhomboid 5 homolog 1
ENSG00000129667	rhomboid 5 homolog 2

Protein Information

Protein Length: 278 | Molecular Weight (kDa): 31.723

UniProt ID(s): A8IWX2, Q8IIM2

PDB ID(s): None

Isoelectric Point: 9.35

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002610	Peptidase S54, rhomboid
InterPro	IPR022764	Peptidase S54, rhomboid domain
PFam	PF01694	Peptidase S54, rhomboid domain
Superfamily	SSF144091	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	13	12	22
disruptive	1	11	10	21
missense	1	11	9	20

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	36	18	19
disruptive	2	35	25	61
missense	2	26	25	48

PlasmoDB Total SNPs: 85

Non-coding: 73 | Synonymous: 9 | Nonsynonymous: 3 | Stop Codon: 0