Product Description: glycerol-3-phosphate dehydrogenase, putative
SignalP Peptide: MLKFFILYFYLIFQAITC
# Transmembrane Domains: 0
EC Numbers: 1.1.1.8 (Glycerol-3-phosphate dehydrogenase (NAD(+)));1.1.1.94 (Glycerol-3-phosphate dehydrogenase (NAD(P)(+)))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Component | GO:0009331 | glycerol-3-phosphate dehydrogenase complex |
| Function | GO:0004367 | glycerol-3-phosphate dehydrogenase [NAD+] activity |
| Process | GO:0006116 | NADH oxidation |
| Process | GO:0006072 | glycerol-3-phosphate metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | not expressed | 0.03 | 0.98 |
| Schizont | expressed | 0.13 | 0.91 |
| Gametocyte | expressed | 0.22 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1114800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.158 | MFS: -2.721 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1431900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0932900)
Modification: Disrupted | RMgm-2642
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIL4
"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.22) with 6PYP (Global RMSD=2.59)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100385
Most Similar Human Ortholog: P21695
TM-align score: 0.97 | RMSD: 1.31
Seq Identity: 0.44 | Length: 348 / 394
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000152642 | glycerol-3-phosphate dehydrogenase 1 like |
| ENSG00000167588 | glycerol-3-phosphate dehydrogenase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 21 | 15 | 26 |
| disruptive | 11 | 35 | 12 | 43 |
| missense | 11 | 35 | 12 | 40 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 36 | 25 | 34 |
| disruptive | 22 | 63 | 32 | 46 |
| missense | 18 | 58 | 32 | 36 |
PlasmoDB Total SNPs: 111
Non-coding: 90 | Synonymous: 10 | Nonsynonymous: 11 | Stop Codon: 0
Protein Length: 394 | Molecular Weight (kDa): 44.875
UniProt ID(s): Q8IIL4
PDB ID(s): None
Isoelectric Point: 8.81
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006109 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminal |
| InterPro | IPR006168 | Glycerol-3-phosphate dehydrogenase, NAD-dependent |
| InterPro | IPR008927 | 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily |
| InterPro | IPR011128 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminal |
| InterPro | IPR017751 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, eukaryotic |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF01210 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminal |
| PFam | PF07479 | Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminal |
| Superfamily | SSF48179 | 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 24330260 | Enzymes involved in plastid-targeted phosphatidic acid synthesis are essentialfor Plasmodium yoelii liver-stage development. | Lindner SE, Sartain MJ, ..., Vaughan AM | 10.1111/mmi.12485 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |