Product Description: palmitoyltransferase DHHC9
SignalP Peptide: N/A
# Transmembrane Domains: 4
EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0070258 | inner membrane pellicle complex |
| Function | GO:0016746 | acyltransferase activity |
| Function | GO:0016409 | palmitoyltransferase activity |
| Function | GO:0019706 | protein-cysteine S-palmitoyltransferase activity |
| Process | GO:0018230 | peptidyl-L-cysteine S-palmitoylation |
| Process | GO:0018345 | protein palmitoylation |
| Process | GO:0006612 | protein targeting to membrane |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.11 | 0.94 |
| Trophozoite | possibly expressed | 0.07 | 0.95 |
| Schizont | expressed | 0.27 | 0.83 |
| Gametocyte | expressed | 0.22 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1115900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.266 | #Insertions: 2
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1243000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0932100)
Modification: Disrupted | RMgm-2637
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIK6
"Best" AlphaFill ligand hit: POV (popc, Local RMSD=0.78) with 6BMS (Global RMSD=4.44)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100499
Most Similar Human Ortholog: Q9UIJ5
TM-align score: 0.78 | RMSD: 3.48
Seq Identity: 0.26 | Length: 264 / 293
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000102383 | zinc finger DHHC-type palmitoyltransferase 15 |
| ENSG00000104219 | zinc finger DHHC-type palmitoyltransferase 2 |
| ENSG00000180776 | zinc finger DHHC-type palmitoyltransferase 20 |
Protein Length: 293 | Molecular Weight (kDa): 34.72
UniProt ID(s): Q8IIK6
PDB ID(s): None
Isoelectric Point: 5.64
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001594 | Palmitoyltransferase, DHHC domain |
| PFam | PF01529 | Palmitoyltransferase, DHHC domain |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 9 | 12 | 11 |
| disruptive | 5 | 10 | 8 | 31 |
| missense | 5 | 10 | 8 | 27 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 29 | 10 | 15 |
| disruptive | 9 | 44 | 36 | 77 |
| missense | 7 | 35 | 31 | 53 |
PlasmoDB Total SNPs: 176
Non-coding: 160 | Synonymous: 10 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 27060339 | Study of Plasmodium falciparum DHHC palmitoyl transferases identifies a role forPfDHHC9 in gametocytogenesis. | Tay CL, Jones ML, ..., Rayner JC | 10.1111/cmi.12599 |
| 30761251 | Plasmodium palmitoylation machinery engineered in E. coli for high-throughputscreening of palmitoyl acyl-transferase inhibitors. | Yadav P, Ayana R, ..., Singh S | 10.1002/2211-5463.12564 |
| 22904317 | The Erf4 subunit of the yeast Ras palmitoyl acyltransferase is required forstability of the Acyl-Erf2 intermediate and palmitoyl transfer to a Ras2substrate | Mitchell DA, Hamel LD, ..., Deschenes RJ | 10.1074/jbc.M112.379297 |
| 27084458 | Palmitoyl transferases have critical roles in the development of mosquito andliver stages of Plasmodium | Hopp CS, Balaban AE, ..., Sinnis P | 10.1111/cmi.12601 |