Product Description: rhoptry neck protein 4
SignalP Peptide: MSSVRFFLCIFLVLFTFIDIVKP
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Component | GO:0032991 | protein-containing complex |
| Component | GO:0020008 | rhoptry |
| Component | GO:1990225 | rhoptry neck |
| Function | GO:0003823 | antigen binding |
| Function | GO:0046812 | host cell surface binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.22 | 0.89 |
| Trophozoite | expressed | 0.37 | 0.81 |
| Schizont | expressed | 3.23 | 0.12 |
| Gametocyte | expressed | 0.13 | 0.92 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1116000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.524 | MFS: -2.539 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0932000)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0932000)
Modification: Mutated | RMgm-5152
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIK5
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 48 | 26 | 61 |
| disruptive | 30 | 144 | 76 | 150 |
| missense | 25 | 123 | 69 | 128 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 19 | 88 | 37 | 66 |
| disruptive | 61 | 258 | 118 | 212 |
| missense | 51 | 217 | 99 | 159 |
PlasmoDB Total SNPs: 370
Non-coding: 124 | Synonymous: 157 | Nonsynonymous: 89 | Stop Codon: 0
Protein Length: 1201 | Molecular Weight (kDa): 135.578
UniProt ID(s): Q8IIK5
PDB ID(s): None
Isoelectric Point: 5.38
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007751 | Domain of unknown function DUF676, lipase-like |
| PFam | PF05057 | Domain of unknown function DUF676, lipase-like |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 16835460 | Plasmodium falciparum AMA1 binds a rhoptry neck protein homologous to TgRON4, acomponent of the moving junction in Toxoplasma gondii. | Alexander DL, Arastu-Kapur S, Dubremetz JF, Boothroyd JC | 10.1128/EC.00040-06 |
| 19442663 | Plasmodium falciparum: genetic and immunogenic characterisation of the rhoptryneck protein PfRON4. | Morahan BJ, Sallmann GB, ..., Waller KL | 10.1016/j.exppara.2009.04.013 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 22177563 | Independent roles of apical membrane antigen 1 and rhoptry neck proteins duringhost cell invasion by apicomplexa. | Giovannini D, Spath S, ..., Menard R | 10.1016/j.chom.2011.10.012 |
| 28371168 | A MA1 and M AEBL are important for Plasmodium falciparum sporozoite infection ofthe liver | Yang ASP, Lopaticki S, ..., Boddey JA | 10.1111/cmi.12745 |
| 30092030 | SURGE complex of Plasmodium falciparum in the rhoptry-neck (SURFIN4.2-RON4-GLURP)contributes to merozoite invasion. | Quintana MDP, Ch'ng JH, ..., Wahlgren M | 10.1371/journal.pone.0201669 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 32817376 | Detection of the Rhoptry Neck Protein Complex in Plasmodium Sporozoites and Its Contribution to Sporozoite Invasion of Salivary Glands | Nozaki M, Baba M, ..., Ishino T | 10.1128/mSphere.00325-20 |
| 37030083 | Plasmodium falciparum rhoptry neck protein 4 has conserved regions mediatinginteractions with receptors on human erythrocytes and hepatocyte membrane | Pulido-Quevedo FA, Arevalo-Pinzon G, ..., Patarroyo MA | 10.1016/j.ijmm.2023.151579 |
| 31552198 | Expression and Localization Profiles of Rhoptry Proteins in Plasmodium bergheiSporozoites | Tokunaga N, Nozaki M, ..., Ishino T | 10.3389/fcimb.2019.00316 |
| 27756311 | Size polymorphism and low sequence diversity in the locus encoding the Plasmodiumvivax rhoptry neck protein 4 (PvRON4) in Colombian isolates | Buitrago SP, Garzon-Ospina D, Patarroyo MA | 10.1186/s12936-016-1563-4 |
| 37272704 | Rhoptry neck protein 4 plays important roles during Plasmodium sporozoiteinfection of the mammalian liver | Baba M, Nozaki M, ..., Ishino T | 10.1128/msphere.00587-22 |
| 30326220 | Deciphering ophthalmic adaptive inhibitors targeting RON4 of Toxoplasma gondii:An integrative in silico approach | Vetrivel U, Nagarajan H | 10.1016/j.lfs.2018.10.022 |
| 32882286 | During host cell traversal and cell-to-cell passage, Toxoplasma gondiisporozoites inhabit the parasitophorous vacuole and posteriorly release densegranule protein-associated membranous trails | Tartarelli I, Tinari A, ..., Spano F | 10.1016/j.ijpara.2020.06.012 |
| 28089547 | Antibody profiles to wheat germ cell-free system synthesized Plasmodiumfalciparum proteins correlate with protection from symptomatic malaria in Uganda | Kanoi BN, Takashima E, ..., Tsuboi T | 10.1016/j.vaccine.2017.01.001 |
| 28848228 | Efficient invasion by Toxoplasma depends on the subversion of host proteinnetworks | Guerin A, Corrales RM, ..., Lebrun M | 10.1038/s41564-017-0018-1 |
| 37197657 | High opsonic phagocytosis activity and growth inhibition of merozoites areassociated with RON4 antibody levels and protect against febrile malaria inGhanaian children | Kyei-Baafour E, Kusi KA, ..., Adu B | 10.3389/fimmu.2023.1161301 |
| 35731833 | The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito andmammalian hosts | Fernandes P, Loubens M, ..., Silvie O | 10.1371/journal.ppat.1010643 |
| 30290224 | Plasmodium RON12 localizes to the rhoptry body in sporozoites | Oda-Yokouchi Y, Tachibana M, ..., Tsuboi T | 10.1016/j.parint.2018.10.001 |
| 29036202 | Toxoplasma gondii induces FAK-Src-STAT3 signaling during infection of host cellsthat prevents parasite targeting by autophagy | Portillo JC, Muniz-Feliciano L, ..., Subauste CS | 10.1371/journal.ppat.1006671 |
| 29081389 | Toxoplasma gondii RON4 binds to heparan sulfate on the host cell surface | Takemae H, Kobayashi K, ..., Kato K | 10.1016/j.parint.2017.10.008 |
| 29488316 | Receptor-ligand and parasite protein-protein interactions in Plasmodium vivax:Analysing rhoptry neck proteins 2 and 4 | Bermudez M, Arevalo-Pinzon G, ..., Patarroyo MA | 10.1111/cmi.12835 |
| 29263399 | RON4(L1) is a new member of the moving junction complex in Toxoplasma gondii | Guerin A, El Hajj H, ..., Lebrun M | 10.1038/s41598-017-18010-9 |
| 29091310 | Design of inhibitory peptide targeting Toxoplasma gondii RON4-human beta-tubulininteractions by implementing structural bioinformatics methods | Vetrivel U, Nagarajan H, Thirumudi I | 10.1002/jcb.26480 |
| 33886601 | Suitability of IgG responses to multiple Plasmodium falciparum antigens asmarkers of transmission intensity and pattern | Kyei-Baafour E, Oppong M, ..., Ofori MF | 10.1371/journal.pone.0249936 |