Product Description: pyridoxine biosynthesis protein PDX2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.4.3.5 (Pyridoxal 5'-phosphate synthase);3.5.1.2 (Glutaminase);4.3.3.6 (Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:1903600 | glutaminase complex |
| Function | GO:0004359 | glutaminase activity |
| Process | GO:0042823 | pyridoxal phosphate biosynthetic process |
| Process | GO:0008614 | pyridoxine metabolic process |
| Process | GO:0000304 | response to singlet oxygen |
| Process | GO:0042819 | vitamin B6 biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | nd | nd |
| Trophozoite | not expressed | nd | nd |
| Schizont | not expressed | nd | nd |
| Gametocyte | not expressed | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1116200.2
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.861 | MFS: -2.264 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A0A4
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.4.3.5 | pyridoxal 5'-phosphate synthase | Phenylglyoxal2,3-Butanedione2,4-pentandioneammonium sulfatediethyldicarbonatepyridoxal 5'-phosphate4-chloromercuribenzoatepyridoxine 5'-phosphatecitrate-phosphate bufferpyridoxamine 5'-phosphate4-Pyridoxic acid phosphatepyridoxaloxime 5'-phosphate... |
| 3.5.1.2 | glutaminase | NH3NEMNaFNaIEDTANH4+PCMBTLCKHgCl2MgCl2CaCl2CuSO4... |
| 4.3.3.6 | pyridoxal 5'-phosphate synthase (glutamine hydrolysing) | acivicinD-erythroseD-erythronhydrazideD-erythrose 4-phosphate2-deoxy-D-ribose 5-phosphate4-phospho-D-erythronhydrazide |
| 1.4.3.5 | pyridoxal 5'-phosphate synthase | Phenylglyoxal2,3-Butanedione2,4-pentandioneammonium sulfatediethyldicarbonatepyridoxal 5'-phosphate4-chloromercuribenzoatepyridoxine 5'-phosphatecitrate-phosphate bufferpyridoxamine 5'-phosphate4-Pyridoxic acid phosphatepyridoxaloxime 5'-phosphate... |
| 3.5.1.2 | glutaminase | NH3NEMNaFNaIEDTANH4+PCMBTLCKHgCl2MgCl2CaCl2CuSO4... |
| 4.3.3.6 | pyridoxal 5'-phosphate synthase (glutamine hydrolysing) | acivicinD-erythroseD-erythronhydrazideD-erythrose 4-phosphate2-deoxy-D-ribose 5-phosphate4-phospho-D-erythronhydrazide |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 11 | 7 | 14 |
| disruptive | 3 | 13 | 8 | 27 |
| missense | 3 | 13 | 8 | 26 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 27 | 6 | 17 |
| disruptive | 6 | 40 | 25 | 54 |
| missense | 6 | 38 | 22 | 45 |
PlasmoDB Total SNPs: 131
Non-coding: 113 | Synonymous: 14 | Nonsynonymous: 3 | Stop Codon: 1
Protein Length: 219 | Molecular Weight (kDa): 24.563
UniProt ID(s): A0A144A0A4, Q8IIK4
Isoelectric Point: 6.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002161 | Pyridoxal 5'-phosphate synthase subunit PdxT/SNO |
| InterPro | IPR021196 | PdxT/SNO family, conserved site |
| InterPro | IPR029062 | Class I glutamine amidotransferase-like |
| PFam | PF01174 | Pyridoxal 5'-phosphate synthase subunit PdxT/SNO |
| Superfamily | SSF52317 | Class I glutamine amidotransferase-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34327152 | Structural Dynamics and Perspectives of Vitamin B6 Biosynthesis Enzymes inPlasmodium: Advances and Open Questions | Barra ALC, Ullah N, ..., Nascimento AS | 10.3389/fcimb.2021.688380 |
| 31626961 | Improved stability of polyclonal antibodies: A case study withlyophilization-conserved antibodies raised against epitopes from the malariaparasite Plasmodium falciparum | Simon N, Sperber C, ..., Friedrich O | 10.1016/j.ejps.2019.105086 |
| 32825141 | Solution Structures and Dynamic Assembly of the 24-Meric Plasmodial Pdx1-Pdx2Complex | Ullah N, Andaleeb H, ..., Wrenger C | 10.3390/ijms21175971 |