About Gene List

PF3D7_1116400 (SEC12)

Genome location: Pf3D7_11_v3:619,245..622,345(-)

Genome classification: Core

Function and Localization

Product Description: guanine nucleotide-exchange factor SEC12

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005789 endoplasmic reticulum membrane
Component GO:0030176 integral component of endoplasmic reticulum membrane
Function GO:0005525 GTP binding
Function GO:0005096 GTPase activator activity
Function GO:0051020 GTPase binding
Process GO:0009306 protein secretion
Process GO:0003400 regulation of COPII vesicle coating

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.39 0.81
Trophozoite expressed 0.61 0.65
Schizont expressed 0.63 0.62
Gametocyte expressed 0.82 0.57

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1116400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.169 | MFS: -2.709 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0931600)

  • Relative Growth Rate: 0.05 ± 0.16
  • Confidence: 5.07

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IIK2

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_132560

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 26 21 31
disruptive 12 44 34 80
missense 12 42 32 71

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 12 58 22 35
disruptive 25 128 80 124
missense 23 108 67 80

PlasmoDB Total SNPs: 110

Non-coding: 79 | Synonymous: 19 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 485 | Molecular Weight (kDa): 56.531

UniProt ID(s): Q8IIK2

PDB ID(s): None

Isoelectric Point: 8.01

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001680 WD40 repeat
InterPro IPR019775 WD40 repeat, conserved site
InterPro IPR036322 WD40-repeat-containing domain superfamily
PFam PF00400 WD40 repeat
Superfamily SSF50978 WD40-repeat-containing domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
18410493 Plasmodium falciparum Sec24 marks transitional ER that exports a model cargo viaa diacidic motif. Lee MC, Moura PA, Miller EA, Fidock DA 10.1111/j.1365-2958.2008.06250.x
32958726 Deletion of PIN4 Suppresses the Protein Transport Defects Caused by sec12-4Mutation in Saccharomyces cerevisiae Murakami-Sekimata A, Sekimata M, ..., Nakano A 10.1159/000509633