Product Description: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 13
EC Numbers: 2.4.99.18 (Dolichyl-diphosphooligosaccharide--protein glycotransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0016021 | integral component of membrane |
| Component | GO:0005887 | integral component of plasma membrane |
| Component | GO:0008250 | oligosaccharyltransferase complex |
| Function | GO:0004579 | dolichyl-diphosphooligosaccharide-protein glycotransferase activity |
| Function | GO:0004576 | oligosaccharyl transferase activity |
| Process | GO:0006487 | protein N-linked glycosylation |
| Process | GO:0006486 | protein glycosylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.21 | 0.87 |
| Schizont | expressed | 0.06 | 0.95 |
| Gametocyte | expressed | 0.18 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1116600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.917 | MFS: -2.76 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0931400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIK0
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.99.18 | dolichyl-diphosphooligosaccharide-protein glycotransferase | EDTARNAiDigitoninoctylglucosideN-benzoyl-Asn-Leu-ThrN-octanoyl-Asn-Leu-ThrN-t-butoxycarbonyl-Asn-Leu-ThrArg-Asn-Gly-(Gly-allyl)-Ala-Val-methyl esterArg-Asn-Gly-(Gly-vinyl)-Ala-Val-methyl esterArg-Asn-Gly-(Gly-allylepoxide)-Ala-Val-methyl esterN-dinitrobenzoyl-Arg-Asn-Ala-epoxyethylglycine-Ala-ValArg-Asn-Gly-(Gly-epoxyethylglycine)-Ala-Val-methylester... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100521
Most Similar Human Ortholog: P46977
TM-align score: 0.94 | RMSD: 2.12
Seq Identity: 0.38 | Length: 688 / 856
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000134910 | STT3 oligosaccharyltransferase complex catalytic subunit A |
| ENSG00000163527 | STT3 oligosaccharyltransferase complex catalytic subunit B |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 49 | 20 | 50 |
| disruptive | 37 | 65 | 36 | 107 |
| missense | 15 | 38 | 27 | 72 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 95 | 25 | 65 |
| disruptive | 58 | 154 | 71 | 174 |
| missense | 24 | 100 | 48 | 102 |
PlasmoDB Total SNPs: 258
Non-coding: 162 | Synonymous: 64 | Nonsynonymous: 32 | Stop Codon: 0
Protein Length: 856 | Molecular Weight (kDa): 101.633
UniProt ID(s): Q8IIK0
PDB ID(s): None
Isoelectric Point: 8.42
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003674 | Oligosaccharyl transferase, STT3 subunit |
| PFam | PF02516 | Oligosaccharyl transferase, STT3 subunit |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 38421550 | Reconstitution and resonance assignments of yeast OST subunit Ost4 and itscritical mutant Ost4V23D in liposomes by solid-state NMR | Chaudhary BP, Struppe J, ..., Mohanty S | 10.1007/s10858-024-00437-8 |
| 33442744 | NMR and MD simulations reveal the impact of the V23D mutation on the function ofyeast oligosaccharyltransferase subunit Ost4 | Chaudhary BP, Zoetewey DL, McCullagh MJ, Mohanty S | 10.1093/glycob/cwab002 |
| 32810478 | Inflammatory conditions promote a switch of oligosaccharyltransferase (OST)catalytic subunit isoform expression | Prados MB, Sica MP, Miranda S | 10.1016/j.abb.2020.108538 |
| 32316603 | Structural Insight into the Mechanism of N-Linked Glycosylation byOligosaccharyltransferase | Mohanty S, Chaudhary BP, Zoetewey D | 10.3390/biom10040624 |
| 32011067 | Comparative and evolutionary analyses of the divergence of plantoligosaccharyltransferase STT3 isoforms | Niu G, Shao Z, ..., Hong Z | 10.1002/2211-5463.12804 |
| 32938717 | Uncoupling the hydrolysis of lipid-linked oligosaccharide from the oligosaccharyltransfer reaction by point mutations in yeast oligosaccharyltransferase | Yamasaki T, Kohda D | 10.1074/jbc.RA120.015013 |
| 32328881 | (1)H, (13)C, (15)N resonance assignments and secondary structure of yeastoligosaccharyltransferase subunit Ost4 and its functionally important mutantOst4V23D | Chaudhary BP, Zoetewey D, Mohanty S | 10.1007/s12104-020-09946-7 |
| 32889138 | Evolutionary considerations of the oligosaccharyltransferase AglB and otheraspects of N-glycosylation across Archaea | Nikolayev S, Cohen-Rosenzweig C, Eichler J | 10.1016/j.ympev.2020.106951 |