Product Description: heat shock protein 101
SignalP Peptide: MTRRYLKYYIFVTLLFFVQVINNVLC
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0097619 | PTEX complex |
| Component | GO:0020007 | apical complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020026 | merozoite dense granule |
| Component | GO:0005634 | nucleus |
| Component | GO:0020003 | symbiont-containing vacuole |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0034605 | cellular response to heat |
| Process | GO:0043335 | protein unfolding |
| Process | GO:0009408 | response to heat |
| Process | GO:0006986 | response to unfolded protein |
| Process | GO:0044053 | translocation of peptides or proteins into host cell cytoplasm |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 2.93 | 0.08 |
| Trophozoite | expressed | 1.08 | 0.47 |
| Schizont | expressed | 0.59 | 0.69 |
| Gametocyte | possibly expressed | 0.77 | 0.60 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1116800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.014 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0931200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0931200)
Modification: Tagged | RMgm-5275
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IIJ8
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.34) with 1QVR (Global RMSD=5.71)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100223
Most Similar Human Ortholog: A0A2R8Y602
TM-align score: 0.61 | RMSD: 4.33
Seq Identity: 0.31 | Length: 282 / 906
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000162129 | caseinolytic mitochondrial matrix peptidase chaperone subunit B |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 52 | 33 | 63 |
| disruptive | 8 | 24 | 16 | 40 |
| missense | 8 | 22 | 14 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 27 | 111 | 32 | 40 |
| disruptive | 15 | 68 | 43 | 127 |
| missense | 13 | 51 | 35 | 92 |
PlasmoDB Total SNPs: 180
Non-coding: 151 | Synonymous: 22 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 906 | Molecular Weight (kDa): 102.874
PDB ID(s): 4IOD, 4IRF, 6E10, 6E11
Isoelectric Point: 9.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001270 | ClpA/B family |
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR004176 | Clp, repeat (R) domain |
| InterPro | IPR018368 | ClpA/B, conserved site 1 |
| InterPro | IPR019489 | Clp ATPase, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR028299 | ClpA/B, conserved site 2 |
| InterPro | IPR036628 | Clp, N-terminal domain superfamily |
| InterPro | IPR041546 | ClpA/ClpB, AAA lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF02861 | Clp, repeat (R) domain |
| PFam | PF07724 | ATPase, AAA-type, core |
| PFam | PF10431 | Clp ATPase, C-terminal |
| PFam | PF17871 | ClpA/ClpB, AAA lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF81923 | Clp, N-terminal domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19536257 | A newly discovered protein export machine in malaria parasites. | de Koning-Ward TF, Gilson PR, ..., Crabb BS | 10.1038/nature08104 |
| 20887733 | The Clp chaperones and proteases of the human malaria parasite Plasmodiumfalciparum. | El Bakkouri M, Pow A, ..., Houry WA | 10.1016/j.jmb.2010.09.051 |
| 22253438 | Biosynthesis, localization, and macromolecular arrangement of the Plasmodiumfalciparum translocon of exported proteins (PTEX). | Bullen HE, Charnaud SC, ..., Gilson PR | 10.1074/jbc.M111.328591 |
| 22970262 | An exported heat shock protein 40 associates with pathogenesis-related knobs inPlasmodium falciparum infected erythrocytes. | Acharya P, Chaubey S, Grover M, Tatu U | 10.1371/journal.pone.0044605 |
| 25043010 | PTEX component HSP101 mediates export of diverse malaria effectors into hosterythrocytes. | Beck JR, Muralidharan V, Oksman A, Goldberg DE | 10.1038/nature13574 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 29487358 | PV1, a novel Plasmodium falciparum merozoite dense granule protein, interactswith exported protein in infected erythrocytes. | Morita M, Nagaoka H, ..., Takashima E | 10.1038/s41598-018-22026-0 |
| 29637707 | The malaria PTEX component PTEX88 interacts most closely with HSP101 at thehost-parasite interface. | Chisholm SA, Kalanon M, ..., de Koning-Ward TF | 10.1111/febs.14463 |
| 30150771 | Malaria parasite translocon structure and mechanism of effector export. | Ho CM, Beck JR, ..., Zhou ZH | 10.1038/s41586-018-0469-4 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31164473 | Uncoupling the Threading and Unfoldase Actions of Plasmodium H SP101 Reveals Differences in Export between Soluble and Insoluble Proteins | Matthews KM, Kalanon M, de Koning-Ward TF | 10.1128/mBio.01106-19 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 35192672 | A revised mechanism for how Plasmodium falciparum recruits and exports proteinsinto its erythrocytic host cell. | Gabriela M, Matthews KM, ..., Gilson PR | 10.1371/journal.ppat.1009977 |
| 37552754 | Knock-sideways by inducible ER retrieval enables a unique approach for studyingPlasmodium-secreted proteins | Fierro MA, Hussain T, Campin LJ, Beck JR | 10.1073/pnas.2308676120 |
| 36238365 | Heme Detoxification Protein (PfHDP) is essential for the hemoglobin uptake andmetabolism in Plasmodium falciparum | Gupta P, Pandey R, ..., Malhotra P | 10.1096/fba.2022-00021 |
| 36445080 | The PTEX Pore Component EXP2 Is Important for Intrahepatic Development during the Plasmodium Liver Stage | Hussain T, Linera-Gonzalez J, ..., Beck JR | 10.1128/mbio.03096-22 |
| 37926971 | Sequence elements within the PEXEL motif and its downstream region modulate PTEX-dependent protein export in Plasmodium falciparum | Gabriela M, Barnes CBG, ..., Gilson PR | 10.1111/tra.12922 |